Fusarium species causing pokkah boeng and their effect on Eldana saccharina Walker (Lepidoptera: Pyralidae).
Fusarium species causing pokkah boeng and their effect on Eldana saccharina Walker (Lepidoptera: Pyralidae).
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发表时间：
2010
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通讯作者：
P. Govender;S. Mcfarlane;R. Rutherford
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作者：
P. Govender;S. Mcfarlane;R. Rutherford
麻花病是一种真菌病，可造成易感品种的严重产量损失。Pokkah boeng是由镰刀菌引起的，但关于所涉及的物种存在一些争论。先前的研究表明，从甘蔗中分离出的一些镰刀菌可能有利于南非甘蔗的一种严重害虫——茎螟Eldana saccharina的发展。本研究的目的是分离和鉴定白粉病的致病菌，并确定分离菌对糖精杆菌发育的影响。8个有代表性的分离株分别接种于麻风易感品种N34和N41。三周后，典型的烟烟症状明显。盆栽试验中接种的分离株和从接种的有症状植物中分离得到的分离株鉴定为糖性F.，增殖F.和笛雅兹F.。将分离株纳入糖精酵母饲料和嗅觉选择试验。与对照组相比，3株分离菌株（2株为F. and diyazi， 1株为F. proliferatum）对糖精双歧杆菌具有拮抗作用，存活幼虫的平均质量显著低于对照组。在本研究中，没有分离株的幼虫质量明显大于对照。嗅觉选择试验结果表明，所有分离株对糖精螟均有吸引力，接种玉米籽粒的幼虫取食量明显高于未接种对照。
Pokkah boeng is a fungal disease that can cause serious yield losses in susceptible varieties. Pokkah boeng is caused by Fusarium, but there is some debate regarding the species involved. Previous research has revealed that some Fusarium species isolated from sugarcane could benefit the development of the stalk borer, Eldana saccharina, a serious pest of sugarcane in South Africa. The aim of this research was to isolate and identify the causal organism of pokkah boeng and to determine the effect of the isolates on the development of E. saccharina. Eight representative isolates were inoculated into pokkah boeng susceptible varieties N34 and N41. Typical pokkah boeng symptoms were evident after three weeks. Isolates inoculated into the pot trial and the isolates obtained from inoculated, symptomatic plants were identified as F. sacchari, F. proliferatum and F. andiyazi. The isolates were included in E. saccharina diet and olfactory choice assays. When compared to controls in the dietary inclusion assays, three isolates were considered to be antagonistic (two identified as F. andiyazi and one as F. proliferatum) to E. saccharina, with the mean mass of surviving larvae being significantly lower than the controls. In this study, no isolates resulted in significantly greater larval mass than the controls. Results from the olfactory choice assays suggested that all isolates were attractive to E. saccharina, with significantly more larvae feeding on inoculated maize kernels than the uninoculated controls.