Ginsenoside-Rg1, one of the major active molecules from Panax ginseng, is a functional ligand of glucocorticoid receptor
Ginsenoside-Rg1, one of the major active molecules from Panax ginseng, is a functional ligand of glucocorticoid receptor
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DOI：
10.1016/s0303-7207(97)00160-3
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发表时间：
1997-10-20
影响因子：
4.1
通讯作者：
Lee, SK
中科院分区：
文献类型：
--
作者：
Lee, YJ;Chung, E;Lee, SK
我们研究了人参成分人参皂苷-Rg1 (G-Rg1) 通过与糖皮质激素受体 (GR) 结合而发挥作用的可能性。 G-Rg1以1-10μM的特异性亲和力竞争[H-3]地塞米松(Dex)与GR的结合，并激活含有糖皮质激素反应元件的荧光素酶报告基因。 G-Rg1 和 Dex 对于这两种效应的剂量依赖性模式几乎相同，尽管对于相同程度的反应需要 G-Rg1 浓度比 Dex 高两到三个数量级。在细胞水平上，FT02B 细胞的生长受到 G-Rg1 和 Dex 的抑制，而 RU486 消除了这两种效应。这些结果表明G-Rg1是GR的功能性配体。 (C) 1997 爱思唯尔科学爱尔兰有限公司
We have examined the possibility that a component of Panax ginseng, ginsenoside-Rg1 (G-Rg1), acts by binding to the glucocorticoid receptor (GR). G-Rg1 competed for [H-3]dexamethasone (Dex) binding to GR with a specific affinity of 1-10 mu M and activated a glucocorticoid responsive element-containing luciferase reporter gene. The dose-dependence patterns of G-Rg1 and Dex for these two effects were nearly identical, although two to three orders of magnitude higher concentration of G-Rg1 than that of Dex was required for the same magnitude of response. At the cellular level, the growth of FT02B cells was suppressed by G-Rg1 as well as by Dex, each of whose effects were abolished by RU486. These results demonstrate that G-Rg1 is a functional ligand of GR. (C) 1997 Elsevier Science Ireland Ltd.