Quantifying the Microvascular Origin of BOLD-fMRI from First Principles with Two-Photon Microscopy and an Oxygen-Sensitive Nanoprobe
Quantifying the Microvascular Origin of BOLD-fMRI from First Principles with Two-Photon Microscopy and an Oxygen-Sensitive Nanoprobe
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DOI：
10.1523/jneurosci.3555-14.2015
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发表时间：
2015-02-25
影响因子：
5.3
通讯作者：
Boas, David A.
中科院分区：
文献类型：
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作者：
Gagnon, Louis;Sakadzic, Sava;Boas, David A.
血液氧合水平依赖性（粗体）对比度广泛用于旨在研究神经元活性的功能磁共振成像（fMRI）研究。但是，粗体信号反映了血容量和氧合的变化，而不是神经元活性本身。因此，了解微观血管行为向宏观BOLD信号的转化是生理知情的非侵入性神经影像的基础。在这里，我们使用氧敏感的两光子显微镜来测量典型的啮齿动物fMRI素体内发生的大胆相关的微血管生理学，并使用这些测量结果预测了第一原理的粗体信号。通过量化人皮层形态折叠引起的粗体信号的变化来说明该方法的预测能力。然后，该框架用于量化各个血管隔室的贡献，以及其他因素对大量信号的贡献，以实现不同的磁力强度和脉冲序列。
The blood oxygenation level-dependent (BOLD) contrast is widely used in functional magnetic resonance imaging (fMRI) studies aimed at investigating neuronal activity. However, the BOLD signal reflects changes in blood volume and oxygenation rather than neuronal activity per se. Therefore, understanding the transformation of microscopic vascular behavior into macroscopic BOLD signals is at the foundation of physiologically informed noninvasive neuroimaging. Here, we use oxygen-sensitive two-photon microscopy to measure the BOLD-relevant microvascular physiology occurring within a typical rodent fMRI voxel and predict the BOLD signal from first principles using those measurements. The predictive power of the approach is illustrated by quantifying variations in the BOLD signal induced by the morphological folding of the human cortex. This framework is then used to quantify the contribution of individual vascular compartments and other factors to the BOLD signal for different magnet strengths and pulse sequences.