Antimicrobial capacity of the freshwater planarians against S. aureus is under the control of Timeless.
Antimicrobial capacity of the freshwater planarians against S. aureus is under the control of Timeless.
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DOI：
10.1080/21505594.2016.1276689
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发表时间：
2017-10-03
期刊：
影响因子：
5.2
通讯作者：
Ghigo, Eric
中科院分区：
文献类型：
--
作者：
Tsoumtsa, Landry Laure;Torre, Cedric;Ghigo, Eric
Planarians是一种非寄生性的扁形虫，对细菌感染有很强的抵抗力。为了更好地了解这种抗性的机制，我们研究了淡水涡虫Schmidtea mediterranea的抗菌反应中昼夜节律机制的作用。我们从S. medialacea作为一个同源的哺乳动物时钟基因蒂姆。我们通过RNA干扰表明，Smed-Tim是在光/暗循环期间Schmidtea mediocorea对抗金黄色葡萄球菌感染的抗微生物活性所必需的。事实上，S。金黄色葡萄球菌感染导致Smed-Tim的表达，其反过来促进Smed-Traf 6和Smed-morn 2，但不促进Smed-p38 MAPK的表达，这两种主要调节真涡虫抗微生物应答。
Planarians, which are non-parasitic flatworms, are highly resistant to bacterial infections. To better understand the mechanisms underlying this resistance, we investigated the role of the circadian machinery in the anti-bacterial response of the freshwater planarian Schmidtea mediterranea. We identified Smed-Tim from S. mediterranea as a homolog of the mammalian clock gene Tim. We showed via RNA interference that Smed-Tim is required for the anti-microbial activities of Schmidtea mediterranea against Staphylococcus aureus infection during the light/dark cycle. Indeed, S. aureus infection leads to the expression of Smed-Tim, which in turn promotes Smed-Traf6 and Smed-morn2, but not Smed-p38 MAPK expression, 2 master regulators of planarian anti-microbial responses.