Extracellular proteins limit the dispersal of biogenic nanoparticles
Extracellular proteins limit the dispersal of biogenic nanoparticles
复制标题
DOI：
10.1126/science.1141064
复制
发表时间：
2007-06-15
期刊：
影响因子：
56.9
通讯作者：
Banfield, Jillian F.
中科院分区：
文献类型：
--
作者：
Moreau, John W.;Weber, Peter K.;Banfield, Jillian F.
高空间分辨率二次离子探针光谱、基于同步辐射的傅里叶变换红外光谱和聚丙烯酰胺凝胶分析表明，蛋白质与生物源硫化锌纳米晶体的球形聚集体密切相关，这是细胞外生物矿化的一个例子。通过合成硫化锌纳米颗粒和代表性氨基酸的实验表明，半胱氨酸在纳米颗粒的快速聚集中起着驱动作用。这些发现表明，微生物来源的细胞外蛋白可以限制纳米颗粒含金属相的扩散，如生物修复的矿物产品，否则这些相可能会通过地下流体流动从它们的来源转移出去。
High-spatial-resolution secondary ion microprobe spectrometry, synchrotron radiation-based Fourier-transform infrared spectroscopy, and polyacrylamide gel analysis demonstrated the intimate association of proteins with spheroidal aggregates of biogenic zinc sulfide nanocrystals, an example of extracellular biomineralization. Experiments involving synthetic zinc sulfide nanoparticles and representative amino acids indicated a driving role for cysteine in rapid nanoparticle aggregation. These findings suggest that microbially derived extracellular proteins can limit the dispersal of nanoparticulate metal-bearing phases, such as the mineral products of bioremediation, that may otherwise be transported away from their source by subsurface fluid flow.