HUMAN CYTOMEGALO-VIRUS RNAS IMMUNOPRECIPITATED BY MULTIPLE SYSTEMIC LUPUS-ERYTHEMATOSUS ANTISERA
HUMAN CYTOMEGALO-VIRUS RNAS IMMUNOPRECIPITATED BY MULTIPLE SYSTEMIC LUPUS-ERYTHEMATOSUS ANTISERA
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DOI：
10.1099/0022-1317-70-9-2383
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发表时间：
1989-09-01
影响因子：
3.8
通讯作者：
KILPATRICK, BA
中科院分区：
文献类型：
--
作者：
LORD, PCW;ROTHSCHILD, CB;KILPATRICK, BA
通过用多名患者的血清进行免疫沉淀，检测了人巨细胞病毒（HCMV）RNA与能与系统性红斑狼疮患者抗体发生反应的核糖核蛋白颗粒的结合情况。一种主要的晚期2.8kb RNA以及由HCMV长重复区编码的几种次要RNA，可被La、Ro自身免疫血清从HCMV感染的细胞中免疫沉淀出来，而被Sm自身免疫血清沉淀出来的量则少得多。通过高分辨率R环作图确定了这些RNA的确切位置，发现其位于0.8093和0.8119图距单位之间。2.8kb RNA是多聚腺苷酸化的，并与多聚核糖体相关，但似乎没有经过剪接。使用正常血清或其他自身免疫血清未观察到免疫沉淀现象。此外，免疫沉淀对这些RNA是特异性的，因为其他丰富的HCMV RNA没有被免疫沉淀。还发现向感染细胞裂解物中加入越来越多的纯化La抗原会抑制La抗血清对2.8kb RNA的免疫沉淀。这些数据表明，特定的HCMV RNA可能与已知参与基因表达转录后调控的细胞核糖核蛋白相互作用。
The association of human cytomegalovirus (HCMV) RNAs with ribonucleoprotein particles that react with antibodies from patients with systemic lupus erythematosus was tested by immunoprecipitation with multiple patients'' sera. A major late 2.8 kb RNA and several minor RNAs encoded by the HCMV long repeat region were immunoprecipitated from HCMV-infected cells by La, Ro and, much less abundantly, Sm autoimmune antisera. The exact location of these RNAs was determined by high resolution R-loop mapping and found to be between 0.8093 and 0.8119 map units. The 2.8 kb RNA is polyadenylated and associated with polysomes but does not appear to be spliced. Immunoprecipitation was not seen using normal or other autoimmune antisera. In addition, immunoprecipitation was specific to these RNAs in that other abundant HCMV RNAs were not immunoprecipitated. It was also found that the addition of increasing amounts of purified La antigen to infected cell lysates inhibited immunoprecipitation of thr 2.8 kb RNA by La antiserum. The data suggest that specific HCMV RNAs may interact with cellular ribonucleoproteins known to be involved in post-transcriptional regulation of gene expression.