The Role of Ecdysteroid 22-Kinase in the Accumulation of Ecdysteroids in Ovary of Silkworm Bombyx mori
The Role of Ecdysteroid 22-Kinase in the Accumulation of Ecdysteroids in Ovary of Silkworm Bombyx mori
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DOI：
10.1111/j.1749-6632.2009.04465.x
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发表时间：
2009-01-01
期刊：
影响因子：
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通讯作者：
Sonobe, Haruyuki
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文献类型：
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作者：
Ito, Yoichi;Sonobe, Haruyuki
在家蚕中，蜕皮甾体22-激酶（EcKinase）已被证明可以催化游离蜕皮甾体转化为无生理活性的蜕皮甾体22-磷酸。首先，EcKinase的系统发育分析表明，许多蛋白质与B同源。桑蜕皮激酶主要存在于蜕皮动物中，包括昆虫和线虫。其次，通过RT-PCR分析结合激光显微切割和免疫组织化学，我们证明了游离蜕皮甾体在B。家蚕卵巢在卵泡细胞中合成并转移到卵母细胞中，在卵母细胞中它们被EcKinase磷酸化，EcKinase的mRNA来源于滋养细胞和卵母细胞本身。
In the silkworm Bombyx mori, ecdysteroid 22-kinase (EcKinase) has been shown to catalyze the conversion of free ecdysteroids to physiologically inactive ecdysteroid 22-phosphates. First, a phylogenetic analysis of EcKinase showed that many proteins homologous to B. mori EcKinase are found mainly in Ecdysozoa, including insects and nematodes. Next, by RT-PCR analysis combined with laser microdissection and by immunohistochemistry, we demonstrated that free ecdysteroids in the B. mori ovary are synthesized in follicle cells and transferred into the oocyte where they are phosphorylated by EcKinase whose mRNA originates from nurse cells and the oocyte itself.