Larvicidal and Growth-Inhibitory Activity of Entomopathogenic Bacteria Culture Fluids Against Aedes aegypti (Diptera: Culicidae)

Larvicidal and Growth-Inhibitory Activity of Entomopathogenic Bacteria Culture Fluids Against Aedes aegypti (Diptera: Culicidae)
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昆虫病原菌培养液对埃及伊蚊(双翅目:蚊科)的杀幼和生长抑制活性

DOI:
10.1093/jee/tow224
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发表时间:
2017
影响因子:
2.2
通讯作者:
Santos da Silva
Santos da Silva
中科院分区:
农林科学2区
文献类型:
--
作者:
da Silva;Undurraga Schwalm;Eugênio Silva;da Costa;Heermann;Santos da Silva

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登革热、基孔肯雅热和寨卡病毒是重要的媒介传播疾病,埃及伊蚊也是重要的媒介传播疾病。是他们的主要发射器由于疾病管理主要基于蚊虫控制策略,因此正在寻找替代和具有成本效益的方法。革兰氏阴性细菌嗜热杆状菌和发光杆状菌与昆虫病原线虫共生,对昆虫幼虫具有高致病性。在我们最近证实了这些细菌的毒性之后。埃及伊蚊幼虫,我们在这里评估了培养液对这种蚊子物种发育的毒性活性。通过将幼虫暴露于不同浓度的P来评估幼虫的敏感性。发光器X.在蚊虫致病性生物测定中,嗜热短杆菌液比嗜热短杆菌液更有效、更稳定。发光的在昆虫暴露于液体后几小时,幼虫开始死亡。此外,X.利培酮持续完全有效4 d。特别是,幼虫死亡率仍高于50%,长达8天。将幼虫暴露于亚致死剂量的X。嗜蚊幼虫延迟化蛹以及成年蚊子的出现,并导致累积幼虫死亡率高于90%的第14天。在这里,我们描述了第一次使用稳定的培养液,因此次级代谢产物ofP。发光体X.因此,嗜热菌作为生物农药防治Ae.埃及未来
Dengue, Chikungunya, and Zika are important vector-borne diseases, andAedes aegyptiL. is their main transmitter. As the disease management is mainly based on mosquito control strategies, the search for alternative and cost-effective approaches is ongoing. The Gram-negative bacteriaXenorhabdus nematophilaandPhotorhabdus luminescensare symbiotically associated with entomopathogenic nematodes and are highly pathogenic for insect larvae. After we have recently confirmed the toxicity of these bacteria inAe. aegyptilarvae, we here evaluated the toxic activity of culture fluids on the development of this mosquito species. Larval susceptibility was assessed by exposing larvae to different concentrations ofP. luminescensorX. nematophilaculture fluids to confirm whether secondary metabolites might cause the mosquitos’ death.Xenorhabdus nematophilaculture fluid was more effective and stable during the mosquito pathogenicity bioassays compared to that ofP. luminescens. Larval mortality started a few hours after exposure of the insects to the fluids. Furthermore, the residual effect of larvicidal activity ofX. nematophilafluid persisted at full efficiency for 4 d. Particularly, larval mortality was still higher than 50% for up to 8 d. Exposure of larvae to a sublethal dose ofX. nematophilafluid delayed pupation as well as emergence of adult mosquitoes and caused cumulative larval mortality higher than 90% by day 14. Here, we describe for the first time the use of stable culture fluids and therefore secondary metabolites ofP. luminescensandX. nematophilaas a promising basis for the use as biopesticide for control ofAe. aegyptiin the future.
埃及伊蚊和白纹伊蚊(双翅目:蚊科):在登革热发生和城市化特征差异化的城市中共存且对替美磷敏感。
DOI: 10.1590/s0037-86822011005000025
发表时间: 2011
影响因子: 2
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发表时间: 2012
期刊: Vector Borne and Zoonotic Diseases
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DOI: 10.1021/np50075a005
发表时间: 1991-05-01
影响因子: 5.1
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DOI: --
发表时间: 2010
期刊: Memórias do Instituto Oswaldo Cruz
影响因子: --
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DOI: --
发表时间: 2011
影响因子: 2
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C. Patil;S. Patil;B. Salunke;R. B. Salunkhe
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