The separation and identification of the lipids of Rhodopseudomonas spheroides
The separation and identification of the lipids of Rhodopseudomonas spheroides
复制标题
球状红假单胞菌脂质的分离与鉴定
DOI：
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发表时间：
1968
期刊：
影响因子：
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通讯作者：
A. Gorchein
中科院分区：
文献类型：
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作者：
A. Gorchein
详细检查的脂质的红球藻进行，因为它被认为这可能提供信息的色素细胞的生物发生。纯化色素的磷脂由磷脂酰乙醇胺（35%），磷脂酰甘油（34%）和磷脂酰胆碱（23%），少量的磷脂酸和心磷脂也被发现。此外，还检测到一种硫脂，也是一种新的脂质含有鸟氨酸。色素细胞中不同磷脂的相对比例，在其他亚细胞组分从色素微生物和在氧气下生长的细胞片段是相似的。然而，“好氧片段”所含的鸟氨酸脂质比色素细胞少得多。在氧气下，在空气中，或在半厌氧条件下，在光下生长的全细胞中的不同磷脂的相对量的比较显示没有显着的差异，该组合物是类似于在色素细胞中发现的。它的结论是，有没有特定的脂质特异性与色素。聚-β-羟基丁酸酯占在苹果酸-谷氨酸培养基上在黑暗中在氧气下生长的细胞干重的35%。有必要在层析前除去该物质，否则它会干扰分离。
Detailed examination of the lipids of Rhodopseudomonas spheroides was undertaken since it was thought this might provide information on the biogenesis of the chromatophores. The phospholipids of purified chromatophores consist of phosphatidylethanolamine (35%), phosphatidylglycerol (34%) and phosphatidylcholine (23%); minor quantities of phosphatidic acid and of cardiolipin were also found. In addition, a sulpholipid was detected and also a new lipid containing ornithine. The relative proportions of the different phospholipids in chromatophores, in other subcellular fractions from pigmented micro-organisms and in fragments from cells grown under oxygen were similar. However, the ‘aerobic fragments’ contained much less ornithine lipid than the chromatophores. Comparison of the relative amounts of the different phospholipids in whole cells grown under oxygen, under air, or under semi-anaerobic conditions in the light showed no marked differences, the composition being similar to that found in chromatophores. It is concluded that there is no particular lipid specifically associated with chromatophores. Poly-β-hydroxybutyrate accounted for as much as 35% of the dry weight of cells grown under oxygen in the dark on malate-glutamate medium. It is necessary to remove this material before chromatography as it otherwise interferes with the separation.