TRANSDUCTION BY PHAGE P1KC IN SALMONELLA-TYPHIMURIUM
TRANSDUCTION BY PHAGE P1KC IN SALMONELLA-TYPHIMURIUM
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DOI：
10.1016/0042-6822(74)90344-4
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发表时间：
1974-01-01
期刊：
影响因子：
3.7
通讯作者：
STOCKER, BAD
中科院分区：
文献类型：
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作者：
ENOMOTO, M;STOCKER, BAD
噬菌体P1 kc以10 - 6至8× 10 - 5/PFU的频率将营养和运动特性转导给一些产生不完全核心LPS的鼠伤寒沙门氏菌LT 2受体，即所有测试的galE类突变体（UDP半乳糖-差向异构酶缺乏），rfaH和rfaG（缺乏半乳糖基-或葡萄糖基-LPS转移酶）和galU（UDP葡萄糖焦磷酸化酶缺乏）和几种产生完全核心LPS的突变体中的一种。H1与flaC、motA和motB的共转导率对于P1 kc为45-65%，而对于P22为0.01-5%。P1 kc共转导约1%的hisC和flaD。从某些弗拉motA受体获得的试验的数目表明，存在于P1 kc转导颗粒中的携带motA的染色体片段中的大多数也携带flaA和flaB，而在P22转导颗粒的情况下，它们中的< 0.4%。噬菌体P1 kc在E. coli K12或志贺菌Shigellaeroteriae 16。由于galE突变而产生半乳糖缺陷型LPS的鼠伤寒受体。观察到trpB+和Meta+的完全转导频率约为1000 MHz。1300（E. coli裂解物）和ca. 110（对于志贺氏菌裂解物），在对照种内杂交中获得的那些。虽然没有获得蜂群，但从不活动的受体中产生的踪迹表明E。大肠杆菌具有flaA、B、C、Q、K、F和其它未鉴定的弗拉基因座的功能等同物，以及S的motA和B的功能等同物。而菌株16只有flaF（或至少是其中的一部分）;这表明志贺氏菌的主要运动基因簇可能被删除。
Phage P1kc transduced nutritional and motility characters, at frequencies of 10− 6 to 8× 10− 5/PFU, to some Salmonella typhimurium LT2 recipients making incomplete core LPS, viz all tested mutants of classes galE (deficient of UDPgalactose-epimerase), rfaH and rfaG (deficient of galactosyl-or glucosyl-LPS transferase) and galU (deficient of UDPglucose pyrophosphorylase) and one only of several mutants making complete core LPS. The rate of cotransduction of H1 with flaC, motA, and motB was 45–65% for P1kc, compared with 0.01–5% for P22. P1kc cotransduced hisC and flaD at about 1%. The numbers of trails obtained from certain fla motA recipients showed that most of the chromosomal fragments carrying motA which are present in P1kc transducing particles also carry flaA and flaB, compared with< 0.4% of them in the case of P22 transducing particles. Phage P1kc grown on E. coli K12 or Shigella dysenteriae 16 was applied to restriction-negative S. typhimurium recipients making galactose-deficient LPS because of galE mutation. Complete transduction of trpB+ and metA+ was observed at frequencies ca. 1 300 (for E. coli lysates) and ca. 1 10 (for Shigella lysates), of those obtained in a control intraspecies cross. Although no swarms were obtained the production of trails from nonmotile recipients showed that E. coli has functional equivalents of flaA, B, C, Q, K, F and other, unidentified, fla loci and also of motA and B of S. typhimurium, whereas strain 16 has only flaF (or, at least, a part of it); this suggests that in Shigella the main cluster of motility genes may be deleted.