A new self-compatibility haplotype in the sweet cherry 'Kronio', S5′, attributable to a pollen-part mutation in the SFB gene
A new self-compatibility haplotype in the sweet cherry 'Kronio', S5′, attributable to a pollen-part mutation in the SFB gene
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DOI：
10.1093/jxb/erm322
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发表时间：
2007-01-01
影响因子：
6.9
通讯作者：
Tobutt, Kenneth R.
中科院分区：
文献类型：
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作者：
Marchese, Annalisa;Boskovic, Radovan I.;Tobutt, Kenneth R.
‘Kronio’是西西里甜樱桃(Prunus Avium)的一个品种，名义上与S5S6不亲和，据报道是自然自交亲和的。在这项工作中，探讨了其自亲和性的原因。自交试验证实了自交亲和性，并提供了用于分析的胚胎；根据S核糖核酸酶和sfb等位基因设计的通用引物进行聚合酶链式反应，结果显示胚胎属于S5S5和S5S6两种类型，表明S 6号花粉失败，而S 5号成功，可能是由于花粉或花柱成分的突变所致。花柱核糖核酸酶分析表明，S-5和S-6都具有S核糖核酸酶活性。对S核糖核酸酶等位基因进行了克隆和测序，并对其编码功能蛋白进行了序列测定。等位基因的克隆和测序表明，S-6是正常的，但S-5在可变区Hva上游存在提前终止密码子，导致蛋白截短。因此，这种自交不亲和性可以归因于S-5的花粉部分突变，命名为S-5‘，这是报道的第一例二倍体甜樱桃自交不亲和性破裂的案例，是由S基因座的自然突变引起的。与S-5‘相关的S核糖核酸酶第二内含子含有一个比S-5的微卫星小的微卫星，设计的扩增微卫星能有效区分S-5和S-5’。对其他一些西西里樱桃的分析表明，S-5‘也存在于西西里品种’Maiolina a RapPu‘中，这被证明是自交亲和的。
'Kronio' is a Sicilian cultivar of sweet cherry (Prunus avium), nominally with the incompatibility genotype S5S6, that is reported to be naturally self-compatible. In this work the cause of its self-compatibility was investigated.. Test selfing confirmed self-compatibility and provided embryos for analysis; PCR with consensus primers designed to amplify S-RNase and SFB alleles showed that the embryos were of two types, S5S5 and S5S6, indicating that S-6 pollen failed, but S-5 succeeded, perhaps because of a mutation in the pollen or stylar component. Stylar RNase analysis indicated active S-RNases for both S-5 and S-6. The S-RNase alleles were cloned and sequenced; and sequences; encode functional proteins. Cloning and sequencing of SFB alleles showed that S-6 was normal but S-5 had a premature stop codon upstream of the variable region HVa resulting in a truncated protein. Therefore, the self-compatibility can be attributed to a pollen-part mutation of S-5, designated S-5', the first reported case of breakdown of self-incompatibility in diploid sweet cherry caused by a natural mutation at the S-locus. The second intron of the S-RNase associated with S-5' contained a microsatellite smaller than that associated with S-5; primers designed to amplify across this microsatellite effectively distinguished S-5 from S-5'. Analysis of some other Sicilian cherries with these primers indicated that S-5' is also present in the Sicilian cultivar 'Maiolina a Rappu', and this proved to be self-compatible.