Calibration-free quantification of absolute oxygen saturation based on the dynamics of photoacoustic signals.
Calibration-free quantification of absolute oxygen saturation based on the dynamics of photoacoustic signals.
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DOI：
10.1364/ol.38.002800
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发表时间：
2013-08-01
期刊：
影响因子：
3.6
通讯作者：
Wang LV
中科院分区：
文献类型：
--
作者：
Xia J;Danielli A;Liu Y;Wang L;Maslov K;Wang LV
光声层析成像（PAT）是一种混合成像技术，具有广泛的临床前和临床应用。基于光声效应，PAT直接测量比光吸收，其是组织固有光吸收系数和局部光通量的乘积。因此，定量PAT，如绝对氧饱和度（sO2）定量，需要了解局部光通量，这只能通过侵入性测量或光传输的复杂建模来估计。在这项工作中，我们规避这一要求，利用在二氧化硫的动态。当可以用多个波长同时监测sO2跃迁时，新方法有效。对于每个波长，利用在不同sO2状态下测量的光声振幅的比率。使用该比率可以消除光学通量的影响，并允许对绝对sO2进行免校准定量。通过体模和体内实验验证了新方法的有效性。
Photoacoustic tomography (PAT) is a hybrid imaging technique that has broad preclinical and clinical applications. Based on the photoacoustic effect, PAT directly measures specific optical absorption, which is the product of the tissue-intrinsic optical absorption coefficient and the local optical fluence. Therefore, quantitative PAT, such as absolute oxygen saturation (sO2) quantification, requires knowledge of the local optical fluence, which can be estimated only through invasive measurements or sophisticated modeling of light transportation. In this work, we circumvent this requirement by taking advantage of the dynamics in sO2. The new method works when the sO2 transition can be simultaneously monitored with multiple wavelengths. For each wavelength, the ratio of photoacoustic amplitudes measured at different sO2 states is utilized. Using the ratio cancels the contribution from optical fluence and allows calibration-free quantification of absolute sO2. The new method was validated through both phantom and in vivo experiments.