EXPRESSION OF A DROSOPHILA GATA TRANSCRIPTION FACTOR IN MULTIPLE TISSUES IN THE DEVELOPING EMBRYOS - IDENTIFICATION OF HOMOZYGOUS LETHAL MUTANTS WITH P-ELEMENT INSERTION AT THE PROMOTER REGION
EXPRESSION OF A DROSOPHILA GATA TRANSCRIPTION FACTOR IN MULTIPLE TISSUES IN THE DEVELOPING EMBRYOS - IDENTIFICATION OF HOMOZYGOUS LETHAL MUTANTS WITH P-ELEMENT INSERTION AT THE PROMOTER REGION
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DOI：
10.1074/jbc.270.42.25150
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发表时间：
1995-10-20
影响因子：
4.8
通讯作者：
TSAI, SF
中科院分区：
文献类型：
--
作者：
LIN, WH;HUANG, LH;TSAI, SF
加塔转录因子是识别核心共有序列WGATAR的DNA结合蛋白。以往的研究表明，加塔因子在脊椎动物组织特异性功能的发育中起着重要的作用。本文报道了一种新的果蝇加塔因子dGATAc的鉴定，它在胚胎中表现出独特的表达模式。dGATAc转录物的局部浓度在不同阶段变化，在6-10阶段的头前区和11-13阶段的后气门、肠道和中枢神经系统中最显著。基于其预测序列，进行DNA结合测定以确认dGATAc基因编码的锌指蛋白能够以预测的特异性结合加塔共有基序。在dGATAc基因启动子区域携带P元件插入的两个独立突变体被鉴定为在胚胎阶段是纯合致死的。使用遗传方案证明，缺乏dGATAC功能会阻碍正常的胚胎发育。我们的研究结果表明，dGATAc蛋白是一个组织特异性转录因子，是至关重要的多器官系统在D。黑腹果蝇
GATA transcription factors are DNA-binding proteins that recognize the core consensus sequence, WGATAR. Previous studies indicated that GATA factors play an important role in the development of tissue-specific functions in vertebrates, Here we report the identification of a new Drosophila melanogaster GATA factor, dGATAc, which displays a distinct expression pattern in embryos. The local concentration of dGATAc transcripts varies at different stages, being most prominent in the procephalic region at stages 6-10 and in the posterior spiracles, the gut, and the central nervous system at stages 11-13. On the basis of its predicted sequence, DNA-binding assays were performed to confirm that the dGATAc gene encodes a zinc finger protein that can bind the GATA consensus motif with predicted specificity. Two independent mutants carrying a P-element insertion at the dGATAc gene promoter region were identified that are homozygous lethal at the embryonic stage. Using a genetic scheme, it was demonstrated that the lack of dGATAc function can block normal embryonic development. Our results suggest that the dGATAc protein is a tissue-specific transcription factor that is vital to the development of multiple organ systems in D. melanogaster.