LOCATION AND ANALYSIS OF MELANINS IN CELL-WALLS OF SOME SOIL FUNGI
LOCATION AND ANALYSIS OF MELANINS IN CELL-WALLS OF SOME SOIL FUNGI
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DOI：
10.1139/m74-212
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发表时间：
1974-01-01
影响因子：
2.8
通讯作者：
GRIFFITHS, DA
中科院分区：
文献类型：
--
作者：
ELLIS, DH;GRIFFITHS, DA
黑色素的电子致密颗粒位于以下几种真菌的菌丝壁、黑附球菌和灰腐质霉的粉孢子壁、炭疽菌的菌核壁以及大丽轮枝菌的微菌核细胞壁内和之间。用KOH从每种真菌中提取黑色素，但由于色素在空气中快速氧化，因此在氮气下进行提取和分析。在UV和IR辐射下以及通过X射线衍射对颜料进行了衍射测定。就其降解产物而言，黑色素的表征表明其性质为吲哚。这些结果进行了比较与其他黑色素提取的动物和植物来源的公布数据。
Electron-dense particles of melanin were located in ultrathin sections of: the hyphal walls ofAmorphotheca resume; the aleuriospore walls ofEpicoccum nigrumandHumicola grisea; the sclerotial walls ofColletotrichum coccodes; and both within and between the microsclerotial cell walls ofVerticillium dahliae. Melanin was extracted with KOH from each of the fungi, but, because of rapid oxidation of the pigment in air, extraction and analysis was carried out under nitrogen. The pigments were examined spectrophotometrically under UV and IR radiation and by means of x-ray diffraction. Characterization of the melanin in terms of its degradation products indicated it to be indolic in nature. These results are compared with published data on other melanins extracted from animal and plant sources.