Delivery rate using cryopreserved oocytes is comparable to conventional in vitro fertilization using fresh oocytes: potential fertility preservation for female cancer patients
Delivery rate using cryopreserved oocytes is comparable to conventional in vitro fertilization using fresh oocytes: potential fertility preservation for female cancer patients
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DOI：
10.1016/j.fertnstert.2009.02.067
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发表时间：
2010-01-15
影响因子：
6.7
通讯作者：
Noyes, Nicole
中科院分区：
文献类型：
--
作者：
Grifo, James A.;Noyes, Nicole
目的：探讨卵母细胞冷冻保存作为一种保存生育能力的方法。在生殖期和青春期进行癌症治疗可能导致不育。以前的生育力保存工作主要集中在胚胎而不是卵母细胞储存上，因为后者被认为效率低下。最近，已经发表了几篇关于移植来自冷冻/解冻卵母细胞的胚胎的健康分娩的大量报道。我们试图建立一个卵母细胞冷冻保存程序在我们的center.Design：23卵母细胞冷冻保存周期进行。收集的卵母细胞冷冻保存无论是缓慢或玻璃化方法。大约1-4个月后，进行解冻/加温、卵胞浆内单精子注射受精和ET的程序化周期;评估周期和妊娠结局。设置：基于大学的生育中心。患者：22名不孕妇女。干预：卵母细胞冷冻保存。主要结局指标：卵母细胞存活、胚胎发育、妊娠结局。结果：卵母细胞存活率、2-原核受精率和囊胚形成率分别为92%、79%和43%。14名妇女怀孕了;一人流产; 10人分娩了13名存活婴儿，3人正在怀孕，正在怀孕/分娩的怀孕率为57%。这一结果是没有统计学差异的周期连续进行年龄匹配的控制，使用新鲜的，nonfrozen自体或供体卵母细胞在一个类似的时间periods. Conclusions（S）：卵母细胞冷冻保存似乎是一个可行的选择，在一些中心的生育力保存。（Fertil Steril（R）2010;93：391-6.（C）2010年由美国生殖医学学会。
Objective: To explore the use of oocyte cryopreservation as a fertility-conserving option. Cancer treatments administered during the reproductive and adolescent years can result in sterility. Previous fertility preservation efforts focused on embryo rather than oocyte storage because the latter was deemed inefficient. Recently, several large reports of healthy births resulting from the transfer of embryos derived from frozen/thawed oocytes have been published. We sought to establish an oocyte cryopreservation program at our center.Design: Twenty-three oocyte cryopreservation cycles were performed. Collected oocytes were cryopreserved by either the slow or the vitrification method. Approximately 1-4 months later,a programmed cycle of thawing/warming, fertilization with intracytoplasmic sperm injection, and ET was performed; cycle and pregnancy Outcomes were assessed.Setting: University-based fertility center.Patient(s): Twenty-two infertile women.Intervention(s): Oocyte cryopreservation.Main Outcome Measure(s): Oocyte survival, embryo development, pregnancy outcomes.Result(s): Oocyte survival, 2-pronuclei fertilization, and blastocyst formation rates were 92%, 79%, and 43%, respectively. Fourteen women became pregnant; one miscarried; 10 have delivered 13 viable infants, and three pregnancies are ongoing for an ongoing/delivered pregnancy rate of 57%. This result was not statistically different from cycles performed consecutively in age-matched controls using fresh, nonfrozen autologous or donor oocytes during a similar time period.Conclusion(s): Oocyte cryopreservation appears to be a viable option for fertility preservation in some centers. (Fertil Steril (R) 2010;93:391-6. (C)2010 by American Society for Reproductive Medicine.)