VACCINATION OF CATTLE WITH THE N-TERMINUS OF L2 IS NECESSARY AND SUFFICIENT FOR PREVENTING INFECTION BY BOVINE PAPILLOMAVIRUS-4
VACCINATION OF CATTLE WITH THE N-TERMINUS OF L2 IS NECESSARY AND SUFFICIENT FOR PREVENTING INFECTION BY BOVINE PAPILLOMAVIRUS-4
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DOI：
10.1006/viro.1995.1392
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发表时间：
1995-08-01
期刊：
影响因子：
3.7
通讯作者：
CAMPO, MS
中科院分区：
文献类型：
--
作者：
CHANDRACHUD, LM;GRINDLAY, GJ;CAMPO, MS
我们之前的研究表明，接种牛乳头瘤病毒-4 （BPV4）的次要结构蛋白L2的牛，在BPV4的攻击下不会发生消化道乳头瘤。对接种L2的动物的B细胞和T细胞应答的分析表明，大部分应答是针对L2的n端和c端，而对中间部分的应答很少。分别用L2的n端或c端接种牛。接种了n末端的动物完全免受病毒攻击，而接种了c末端的动物则没有。通过合成横跨整个n端的重叠肽进一步分析，在101-120氨基酸上定位了B细胞免疫优势表位。该表位被所有接种疫苗的动物识别。(C) 1995学术出版社，Inc。
We have previously shown that cattle vaccinated with L2, the minor structural protein of bovine papillomavirus-4 (BPV4), do not develop alimenlary papillomas upon challenge with BPV-4. Analysis of the B and T cell response in L2-vaccinated animals showed that the majority of the response was directed against the N-terminus and C-terminus of L2 with little response against the middle portion. Cattle were vaccinated with the N-terminus or the C-terminus of L2. The animals vaccinated with the N-terminus were completely protected from viral challenge, whereas the animals vaccinated with the C-terminus were not. Further analysis with synthetic overlapping peptides spanning the entire N-terminus mapped a B cell immunodominant epitope at amino acid 101-120. This epitope was recognised by all vaccinated animals. (C) 1995 Academic Press, Inc.