Carotenoid diversity in cultivated citrus is highly influenced by genetic factors
Carotenoid diversity in cultivated citrus is highly influenced by genetic factors
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DOI：
10.1021/jf0526644
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发表时间：
2006-06-14
影响因子：
6.1
通讯作者：
Ollitrault, Patrick
中科院分区：
文献类型：
--
作者：
Fanciullino, Anne-Laure;Dhuique-Mayer, Claudie;Ollitrault, Patrick
柑橘类水果是复杂的类胡萝卜素来源，据报道该属中含有100多种色素。为了解柑橘类胡萝卜素组分多样性的来源，对8种栽培柑橘的25个基因型进行了分析。成熟果实的果汁提取物通过高效液相色谱法使用C-30柱进行分析。25个柑橘基因型呈现出不同的类胡萝卜素谱，分离出25种不同的化合物。统计分析显示，类胡萝卜素组合物的基因型的强烈影响。两种类型的基因型进行分类：定性数据和定量数据，分别。结果表明，类胡萝卜素组分的变异性种间大于种内。两个类胡萝卜素，顺式紫黄质和β-隐黄质，强烈确定定性数据的分类，这也是与以前的柑橘品种分类。这些发现提供了证据，作为其他表型性状，一般的栽培柑橘的进化是柑橘品种之间的类胡萝卜素多样性的组织的主要因素。据作者所知，这是第一个将类胡萝卜素组成的多样性与柑橘遗传多样性联系起来的研究。这些结果导致提出的主要生物合成步骤中涉及的差异类胡萝卜素积累。可能的调节机制也进行了讨论。
Citrus fruits are complex sources of carotenoids with more than 100 kinds of pigments reported in this genus. To understand the origin of the diversity of carotenoid compositions of citrus fruit, 25 genotypes that belong to the 8 cultivated Citrus species were analyzed. Juice extracts of mature fruit were analyzed by high-performance liquid chromatography using a C-30 column. The 25 citrus genotypes presented different carotenoid profiles with 25 distinct compounds isolated. Statistical analyses revealed a strong impact of genotype on carotenoid compositions. Two kinds of classifications of genotypes were performed: on qualitative data and on quantitative data, respectively. The results showed that variability in carotenoid compositions was more interspecific than intraspecific. Two carotenoids, cis-violaxanthin and the beta-cryptoxanthin, strongly determined the classification on qualitative data, which was also in agreement with previous citrus variety classifications. These findings provide evidence that, as for other phenotypical traits, the general evolution of cultivated Citrus is the main factor of the organization of carotenoid diversity among citrus varieties. To the authors' knowledge this is the first study that links the diversity of carotenoid composition to the citrus genetic diversity. These results lead to the proposed major biosynthetic steps involved in the differential carotenoid accumulation. Possible regulation mechanisms are also discussed.