Occurrence of natural dixenic associations between the symbiont Photorhabdus luminescens and bacteria related to Ochrobactrum spp. in tropical entomopathogenic Heterorhabditis spp. (Nematoda, Rhabditida)
Occurrence of natural dixenic associations between the symbiont Photorhabdus luminescens and bacteria related to Ochrobactrum spp. in tropical entomopathogenic Heterorhabditis spp. (Nematoda, Rhabditida)
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DOI：
10.1099/00221287-146-3-709
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发表时间：
2000-03-01
期刊：
影响因子：
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通讯作者：
Boemare, N
中科院分区：
文献类型：
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作者：
Babic, I;Fischer-Le Saux, M;Boemare, N
从昆虫病原线虫印度异小杆线虫中分离出与发光小杆菌阿克氏亚种共生的细菌。从3个不同的加勒比海岛屿采集的线虫样品中分离到14个细菌分离物，通过常规的表型试验、限制性片段长度多态性和PCR扩增的16S rRNA基因序列分析对其进行了表征分离株分为三种基因型，每种基因型与一个加勒比岛屿有关。表型特征和16SrDNA分析表明，来自瓜德罗普的发光杆菌属细菌与人苍白杆菌（Ochrobactrum anthropi）亲缘关系较近，来自多米尼加共和国和波多黎各的发光杆菌属细菌与中间苍白杆菌（Ochrobactrum intermedium）亲缘关系较近。自苍白杆菌属（Ochrobactrum spp.）被认为是人类的机会主义病原体，大量生产用于生物防治的昆虫病原线虫需要严格警惕。
Bacteria naturally associated with the symbiont Photorhabdus luminescens subsp, akhurstii were isolated from the entomopathogenic nematode Heterorhabditis indica. Bacterial isolates distinct from P, luminescens subsp, akhurstii were obtained from 33 % of the samples, Fourteen bacterial isolates, from nematodes collected from three different Caribbean islands, were characterized by conventional phenotypic tests, restriction fragment length polymorphism and sequence analyses of PCR-amplified 16S rRNA genes (16S rDNAs), Isolates were grouped into three genotypes, each one being associated with one Caribbean island. Phenotypic characteristics and 16S rDNA analysis showed that the Photorhabdus-associated bacteria were closely related to Ochrobactrum anthropi for the group from Guadeloupe, and to Ochrobactrum intermedium for the two groups from the Dominican Republic and Puerto Rico, No pathogenicity of the Ochrobactrum spp, to the insects Galleria mellonella and Spodoptera littoralis (Lepidoptera) was detected. Since Ochrobactrum spp. are considered as human opportunist pathogens, the mass production of entomopathogenic nematodes for biological control requires strict vigilance.