Differences in steroid 5α-reductase iso-enzymes expression between normal and pathological human prostate tissue
Differences in steroid 5α-reductase iso-enzymes expression between normal and pathological human prostate tissue
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正常与病理人前列腺组织中类固醇5α-还原酶同工酶表达的差异
DOI：
10.1016/s0960-0760(99)00030-8
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
P. Martin
中科院分区：
文献类型：
--
作者：
C. Iehlé;F. Radvanyi;S. Medina;L. Ouafik;H. Gérard;D. Chopin;J. Raynaud;P. Martin
本研究采用半定量逆转录聚合酶链式反应和原位杂交技术，研究了5-α-还原酶(5-α-R)1和2同工酶在人前列腺增生症和前列腺癌组织中的表达水平和细胞特异性表达模式。原位杂交结果表明，5α-R1m RNA主要在上皮细胞表达，间质细胞几乎不表达，而5α-R2m RNA在上皮和间质细胞中均有表达。对正常前列腺、BPH组织和肝脏不同部位的总RNA进行了半定量RT-PCR。我们发现5个α-R1mRNA和5个α-R2mRNAs在正常前列腺各区的表达基本相同。在前列腺增生症组织中，与正常前列腺组织相比，5α-R1和5α-R2的mRNAs表达略有增加，但显著增加。在前列腺癌组织中，5α-R1mRNA的表达高于正常前列腺和增生性前列腺组织，但5α-R2mRNA的表达水平与正常前列腺不同区段的差异无统计学意义。在肝脏中，5α-R2mRNA的表达水平与良性前列腺增生症相似，但5α-R1mRNA的表达水平比良性前列腺增生症高10倍。5-α-R同工酶在前列腺组织中的表达增加可能在该病的发病和/或维持中起重要作用。
We studied the expression level and cell-specific expression patterns of 5α-reductase (5α-R) types 1 and 2 iso-enzymes in human hyperplastic and malignant prostate tissue by semi-quantitative RT-PCR and in situ hybridisation analyses. In situ hybridisation established that 5α-R1 mRNA is preferentially expressed by epithelial cells and little expressed by stromal cells whereas 5α-R2 mRNA is expressed by both epithelium and stroma. Semi-quantitative RT-PCR has been performed on total RNA from different zones of normal prostate, BPH tissues and liver. We found that 5α-R1 and 5α-R2 mRNAs expression was near the same in all zones of normal prostate. In BPH tissue, 5α-R1 and 5α-R2 mRNAs expression was slightly but significantly increased, when it was compared to the levels recorded for normal prostate. In cancer samples, 5α-R1 mRNA expression was higher than in normal and hyperplastic prostate but the level of 5α-R2 mRNA was not statistically different from that observed in the different zones of normal prostate. In liver, 5α-R2 mRNA level was similar to that measured in BPH but 5α-R1 mRNA expression was ten times higher. The increase observed in 5α-R isoenzymes expression in BPH tissue could play an important role in the pathogenesis and/or maintenance of the disease.
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类固醇 5-α-还原酶-2缺乏症的分子遗传学研究
影响因子：
15.9
作者：
THIGPEN, AE;DAVIS, DL;RUSSELL, DW
通讯作者：
RUSSELL, DW
影响因子：
15.9
作者：
THIGPEN, AE;SILVER, RI;RUSSELL, DW
通讯作者：
RUSSELL, DW
人类类固醇 5 α-还原酶基因和假基因的表征和染色体作图以及小鼠同源物的作图。
影响因子：
4.4
作者：
Jenkins,EP;Hsieh,CL;Milatovich,A;Normington,K;Berman,DM;Francke,U;Russell,DW
通讯作者：
Russell,DW
影响因子：
15.9
作者：
JENKINS, EP;ANDERSSON, S;RUSSELL, DW
通讯作者：
RUSSELL, DW