Transcriptional profiling of the hyperthermophilic methanarchaeon Methanococcus jannaschii in response to lethal heat and non-lethal cold shock
Transcriptional profiling of the hyperthermophilic methanarchaeon Methanococcus jannaschii in response to lethal heat and non-lethal cold shock
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DOI：
10.1111/j.1462-2920.2005.00751.x
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发表时间：
2005-06-01
影响因子：
5.1
通讯作者：
Clark, DS
中科院分区：
文献类型：
--
作者：
Boonyaratanakornkit, BB;Simpson, AJ;Clark, DS
超嗜热甲烷古菌jannaschii甲烷球菌的最适生长温度为85℃至65℃和95℃，温度冲击导致转录响应的方向（热冲击还是冷冲击）和是否致死性不同。95℃致死性热休克的具体结果包括编码伴侣蛋白的基因上调和编码H+转运ATP合成酶亚基的基因下调。一个编码预折叠蛋白α亚基的基因也被上调，这可能是m.j annaschii蛋白质加工途径中的一个新元件。在65摄氏度的冷休克中观察到非常不同的反应，包括编码RNA解旋酶的基因和其他参与转录和翻译的基因的上调，以及编码蛋白酶和运输蛋白的基因的上调。同样上调的还有一个编码18 kDa fkbp型PPIase的基因，该基因可能促进蛋白质在低温下折叠。转录谱分析还揭示了几种对温度胁迫条件有反应的假设蛋白质。
Temperature shock of the hyperthermophilic methanarchaeon Methanococcus jannaschii from its optimal growth temperature of 85 degrees C to 65 degrees C and 95 degrees C resulted in different transcriptional responses characteristic of both the direction of shock (heat or cold shock) and whether the shock was lethal. Specific outcomes of lethal heat shock to 95 degrees C included upregulation of genes encoding chaperones, and downregulation of genes encoding subunits of the H+ transporting ATP synthase. A gene encoding an alpha subunit of a putative prefoldin was also upregulated, which may comprise a novel element in the protein processing pathway in M. jannaschii. Very different responses were observed upon cold shock to 65 degrees C. These included upregulation of a gene encoding an RNA helicase and other genes involved in transcription and translation, and upregulation of genes coding for proteases and transport proteins. Also upregulated was a gene that codes for an 18 kDa FKBP-type PPIase, which may facilitate protein folding at low temperatures. Transcriptional profiling also revealed several hypothetical proteins that respond to temperature stress conditions.