WEIGHT-REDUCING EFFECTS OF THE PLASMA-PROTEIN ENCODED BY THE OBESE GENE
WEIGHT-REDUCING EFFECTS OF THE PLASMA-PROTEIN ENCODED BY THE OBESE GENE
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DOI：
10.1126/science.7624777
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发表时间：
1995-07-28
期刊：
影响因子：
56.9
通讯作者：
FRIEDMAN, JM
中科院分区：
文献类型：
--
作者：
HALAAS, JL;GAJIWALA, KS;FRIEDMAN, JM
Ob基因座的基因产物在体重调节中很重要。该ob产物在小鼠和人的血浆中以16千道尔顿的形式存在，但在C57BL/6job/ob小鼠的血浆中检测不到。糖尿病(Db)小鼠血浆中这种蛋白的水平升高，这是一种被认为对ab的影响具有抵抗力的突变。每天给ob/ob小鼠或人重组ob蛋白注射，治疗2周后体重减轻30%，没有明显的毒性，但对db/db小鼠没有影响。这种蛋白质减少了肥胖/肥胖小鼠的食物摄入量，增加了能量消耗。每天给野生型小鼠注射两次这种小鼠蛋白质后，体重持续下降12%，食物摄入量减少，体脂从12.2%减少到0.7%。这些数据表明，OB蛋白具有调节体内脂肪储存的内分泌功能。
The gene product of the ob locus is important in the regulation of body weight. The ob product was shown to be present as a 16-kilodalton protein in mouse and human plasma but was undetectable in plasma from C57BL/6J ob/ob mice. Plasma levels of this protein were increased in diabetic (db) mice, a mutant thought to be resistant to the effects of ab. Daily intraperitoneal injections of either mouse or human recombinant OB protein reduced the body weight of ob/ob mice by 30 percent after 2 weeks of treatment with no apparent toxicity but had no effect on db/db mice. The protein reduced food intake and increased energy expenditure in ob/ob mice. Injections of wild-type mice twice daily with the mouse protein resulted in a sustained 12 percent weight loss, decreased food intake, and a reduction of body fat from 12.2 to 0.7 percent. These data suggest that the OB protein serves an endocrine function to regulate body fat stores.