The quest for a message: budding yeast, a model organism to study the control of pre-mRNA splicing
The quest for a message: budding yeast, a model organism to study the control of pre-mRNA splicing
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DOI：
10.1093/bfgp/elp002
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发表时间：
2009-01-01
期刊：
影响因子：
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通讯作者：
Vilardell, Josep
中科院分区：
文献类型：
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作者：
Meyer, Markus;Vilardell, Josep
在pre-mRNA剪接过程中内含子的去除是基因表达的一个关键过程，了解其在单基因和基因组水平上的控制是生物学的巨大挑战之一。剪接发生在一个被称为剪接体的动态的大型核糖核蛋白复合体中。结合遗传学和生物化学，酿酒酵母提供了其机制的见解，包括rna -蛋白相互作用的调控。最近的全基因组分析表明，即使在具有相对简单的内含子结构的生物体（如酵母）中，受调节的剪接也广泛存在并且具有生物学相关性。此外，在这种模式生物中，在基因组水平上剪接调节协调的可能性正在变得清晰。这应该提供一个有价值的系统来接近调控剪接在基因组表达中的作用的复杂问题。
Removal of introns during pre-mRNA splicing is a critical process in gene expression, and understanding its control at both single-gene and genomic levels is one of the great challenges in Biology. Splicing takes place in a dynamic, large ribonucleoprotein complex known as the spliceosome. Combining Genetics and Biochemistry, Saccharomyces cerevisiae provides insights into its mechanisms, including its regulation by RNAprotein interactions. Recent genome-wide analyses indicate that regulated splicing is broad and biologically relevant even in organisms with a relatively simple intronic structure, such as yeast. Furthermore, the possibility of coordination in splicing regulation at genomic level is becoming clear in this model organism. This should provide a valuable system to approach the complex problem of the role of regulated splicing in genomic expression.