Isolation and identification of cancer stem cells from a side population of a human hepatoblastoma cell line, HuH-6 clone-5
Isolation and identification of cancer stem cells from a side population of a human hepatoblastoma cell line, HuH-6 clone-5
复制标题
DOI：
10.1007/s00383-010-2719-x
复制
发表时间：
2011-01-01
影响因子：
1.8
通讯作者：
Satomi, Akira
中科院分区：
文献类型：
--
作者：
Hayashi, Shinichi;Fujita, Keiko;Satomi, Akira
研究目的人们认为，即使体内存在少量肿瘤细胞，也可能以类似的方式增加每个肿瘤细胞，并可能使疾病继续发展。然而，只有百分之几的这种肿瘤细胞存在于癌组织中。它们被称为“癌症干细胞（CSCs）”。如果找到一种消灭CSC的替代方法，它将极大地阻止复发和转移。方法采用SP法从肝母细胞瘤细胞中提取CSC候选细胞群。结果SP细胞接种小鼠后可形成肿瘤，而非SP细胞接种小鼠后未形成肿瘤。结论SP法可从肝母细胞瘤细胞中分离出肿瘤干细胞样细胞。体内实验证明SP组分细胞接种到小鼠体内可自我复制，并鉴定出肿瘤干细胞样细胞的存在。
Purpose It has been thought that the persistence of even a small number of tumor cells in the body may increase each tumor cell in a similar manner and may allow the disease to proceed. However, only a few percent of such tumor cells exist in cancerous tissue. They are called "cancer stem cells (CSCs)''. If an alternative method of annihilating CSCs is found, it will greatly deter relapse and metastasis. We attempted to identify and separate CSCs in hepatoblastoma aiming to develop a new therapy for hepatoblastoma.Methods The side population (SP) method was used as an indicator when extracting the CSC candidate group from the hepatoblastoma cells. The SP cells and non-SP cells were studied for tumourigenesis.Results Although tumors were formed when SP fraction cells were inoculated into mice, tumor formation was not observed in non-SP cells. SP cells had higher tumor formation ability compared to non-SP cells.Conclusion Cancer stem-like cells were separated by the SP fraction method from hepatoblastoma cells. The in vivo experiment proved that SP fraction cells inoculated into mice were self-replicated, and the existence of cancer stem-like cells was identified.