Progressive alterations in microstructural organization and biomechanical response in the ApoE mouse model of aneurysm.
Progressive alterations in microstructural organization and biomechanical response in the ApoE mouse model of aneurysm.
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DOI：
10.4161/biom.24648
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发表时间：
2013-07
期刊：
影响因子：
--
通讯作者：
Vande Geest JP
中科院分区：
文献类型：
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作者：
Haskett D;Azhar M;Utzinger U;Vande Geest JP
AAA是一种复杂的疾病，导致肾下主动脉的局部扩张，多年来发展。在人类中很难获得这种疾病的纵向信息，因此小鼠模型已越来越多地用于研究AAAs的发展。本研究的目的是确定疾病进展期间ApoE−/− AngII小鼠动脉瘤模型中生物力学反应和纤维微观结构发生的任何变化。在第14和28天采集成年ApoE−/− AngII输注小鼠和野生型对照小鼠沿着。切除主动脉并同时测试双轴机械反应和ECM组织。将数据集拟合到Fung型本构模型以给出峰值应变和刚度值。对来自双光子显微镜的图像进行定量，以评估优选的纤维排列和纤维取向程度。生物力学结果发现，14 d时存在的显著差异在28 d沿着恢复正常，纤维取向和分散发生显著变化，表明血管壁内发生重塑。一些正常生物力学功能的恢复，以及微观结构中发生的持续变化表明，在初始动脉瘤形成后，ApoE−/− AngII输注模型中发生了恢复性反应。
AAA is a complex disease that leads to a localized dilation of the infrarenal aorta that develops over years. Longitudinal information in humans has been difficult to obtain for this disease, therefore mouse models have become increasingly used to study the development of AAAs. The objective of this study was to determine any changes that occur in the biomechanical response and fiber microstructure in the ApoE−/− AngII mouse model of aneurysm during disease progression. Adult ApoE−/− AngII infused mice along with wild-type controls were taken at 14 and 28 d. Aortas were excised and tested simultaneously for biaxial mechanical response and ECM organization. Data sets were fit to a Fung-type constitutive model to give peak strains and stiffness values. Images from two photon microscopy were quantified in order to assess the preferred fiber alignment and degree of fiber orientation. Biomechanical results found significant differences that were present at 14 d had returned to normal by 28 d along with significant changes in fiber orientation and dispersion indicating remodeling occurring within the aneurysmal wall. This return of some of the normal biomechanical function, in addition the continuing changes that occur in the microstructure suggest a restorative response that occurs in the ApoE−/− AngII infused model after the initial aneurysm formation.