Allostery and the lac Operon
Allostery and the lac Operon
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DOI：
10.1016/j.jmb.2013.03.003
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发表时间：
2013-07-10
影响因子：
5.6
通讯作者：
Lewis, Mitchell
中科院分区：
文献类型：
--
作者：
Lewis, Mitchell
调节基因表达的能力对于控制细胞中的代谢事件是必不可少的。蛋白质的功能就像分子开关一样，对环境的波动做出反应，以维持体内平衡。Jacob和Monod提出的操纵子模型为基因表达如何调控提供了令人信服的描述。调节的分子机制与变构转换理论紧随其后。在过去的半个世纪里，乳糖操纵子和变构模型的细节已经使用遗传，生物化学和结构技术进行了测试。值得注意的是，50年前最初提出的原则基本上没有改变。操纵子模型和变构转换理论是分子生物学中两个最深刻的发现。(c)2013由Elsevier Ltd.出版
The ability to regulate gene expression is essential for controlling metabolic events in a cell. Proteins that function like molecular switches respond to fluctuations in the environment to maintain homeostasis. The operon model, proposed by Jacob and Monod, provides a cogent depiction for how gene expression is regulated. A molecular mechanism for the regulation followed shortly with the theory for allosteric transition. Over the past half-century, the details of the lac operon and the allosteric model have been tested using genetic, biochemical, and structural techniques. Remarkably, the principles originally put forward 50 years ago remain essentially unchanged. Models for the operon and the theory of allosteric transitions are two of the most profound discoveries of molecular biology. (c) 2013 Published by Elsevier Ltd.