Novel macrolide resistance module carried by the IncP-1β resistance plasmid pRSB111, isolated from a wastewater treatment plant
Novel macrolide resistance module carried by the IncP-1β resistance plasmid pRSB111, isolated from a wastewater treatment plant
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DOI：
10.1128/aac.00802-06
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发表时间：
2007-02-01
影响因子：
4.9
通讯作者：
Schlueter, Andreas
中科院分区：
文献类型：
--
作者：
Szczepanowski, Rafael;Krahn, Irene;Schlueter, Andreas
大环内酯类耐药质粒pRSB 111是从废水处理厂最终流出物中的细菌中分离得到的。47-kb质粒在由假单胞菌属菌株B13携带时赋予对阿奇霉素、克拉霉素、红霉素、罗红霉素和泰乐菌素的抗性，并且非常类似于原型IncP-1 β质粒pB 3，其先前从废水处理厂的活性污泥细菌群落中分离。这两种质粒的不同之处在于它们的辅助区，位于接合转移模块基因traC的下游。对pRSB 111辅助区的核苷酸序列分析表明，它含有一个新的大环内酯类药物抗性模块，该模块由mphR（E）、mph（E）和meptin（E）基因组成，它们分别编码转录调节因子、大环内酯类药物磷酸转移酶和跨膜转运蛋白。对大环内酯类耐药模块的单个基因的贡献的分析显示，mph（E）和mglutamine（E）是高水平大环内酯类耐药所必需的。抗性基因侧翼有两个插入序列，即1 SPa 15和ISRSB 111。两个截短的转座因子，IS 6100和Tn 3样转座子的残余物，被确定在ISRSB 111附近。pRSB 111的辅助元件显然取代了姐妹质粒pB 3上存在的Tn 402样元件，正如pRSB 111上Tn 402特异性末端反向重复序列的保守性所表明的那样。
The macrolide resistance plasmid pRSB111 was isolated from bacteria residing in the final effluents of a wastewater treatment plant. The 47-kb plasmid confers resistance to azithromycin, clarithromycin, erythromycin, roxithromycin, and tylosin when it is carried by Pseudomonas sp. strain B13 and is very similar to prototype IncP-1 beta plasmid pB3, which was previously isolated from an activated-sludge bacterial community of a wastewater treatment plant. The two plasmids differ in their accessory regions, located downstream of the conjugative transfer module gene traC. Nucleotide sequence analysis of the pRSB111 accessory region revealed that it contains a new macrolide resistance module composed of the genes mphR(E), mph(E), and mrx(E), which putatively encode a transcriptional regulator, a macrolide phosphotransferase, and a transmembrane transport protein, respectively. Analysis of the contributions of the individual genes of the macrolide resistance module revealed that mph(E) and mrx(E) are required for high-level macrolide resistance. The resistance genes are flanked by two insertion sequences, namely, 1SPa15 and ISRSB111. Two truncated transposable elements, IS6100 and remnants of a Tn3-like transposon, were identified in the vicinity of ISRSB111. The accessory element of pRSB111 apparently replaced the Tn402-like element present on the sister plasmid, pB3, as suggested by the conservation of Tn402-specific terminal inverted repeats on pRSB111.