OsCERK2/OsRLK10, a homolog of OsCERK1, has a potential role for chitin-triggered immunity and arbuscular mycorrhizal symbiosis in rice.
OsCERK2/OsRLK10, a homolog of OsCERK1, has a potential role for chitin-triggered immunity and arbuscular mycorrhizal symbiosis in rice.
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OsCERK2/OsRLK10是OsCERK1的同源物，在水稻几丁质触发免疫和丛枝菌根共生中具有潜在作用。
DOI：
10.5511/plantbiotechnology.21.1222a
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发表时间：
2022-06
影响因子：
1.6
通讯作者：
Kana Miyata;Shun Hasegawa;E. Nakajima;Y. Nishizawa;Kota Kamiya;Hirotaka Yokogawa;Subaru Shirasaka
中科院分区：
文献类型：
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作者：
Kana Miyata;Shun Hasegawa;E. Nakajima;Y. Nishizawa;Kota Kamiya;Hirotaka Yokogawa;Subaru Shirasaka
在水稻中，溶素基序 (LysM) 受体样激酶 OsCERK1 最初被认为是几丁质触发免疫的必需分子，在丛枝菌根 (AM) 共生中发挥着关键作用。正如我们之前报道的，虽然 AM 定植在接种后 2 周（WAI）时很大程度上受到抑制，但在 oscerk1 突变体中，在 5 WAI 时观察到丛枝。相反，大多数缺陷共同共生信号通路的突变植物没有表现出丛枝形成。关于oscerk1这种特征表型的原因，我们推测OsRLK10（OsCERK1的推定旁系同源物）可能在AM共生中具有冗余功能。这两个基因的蛋白质序列高度保守，估计基因复制发生在1.5亿年前。在这里，我们证明了 OsCERK2/OsRLK10 在 oscerk1 敲除突变体中诱导 AM 定植和几丁质触发的活性氧产生，与 OsCERK1 类似。 oscerk2 突变体在 5 WAI 时表现出轻微但显着的 AM 定植减少，表明 OsCERK2 对 AM 共生的贡献。然而，oscerk2;oscerk1 双敲除突变体在 5 WAI 时产生丛枝，与 oscerk1 突变体相似，表明 OsCERK1 和 OsCERK2 的冗余并不能解释 oscerk1 在 5 WAI 时的菌根定植。这些结果表明，OsCERK2 具有调节几丁质触发的免疫和 AM 共生的潜力，并且至少部分地促进水稻中的 AM 共生，尽管 OsCERK2 的贡献似乎弱于 OsCERK1。
In rice, the lysin motif (LysM) receptor-like kinase OsCERK1, originally identified as the essential molecule for chitin-triggered immunity, plays a key role in arbuscular mycorrhizal (AM) symbiosis. As we previously reported, although AM colonization was largely repressed at 2 weeks after inoculation (WAI), arbuscules were observed at 5 WAI in oscerk1 mutant. Conversely, most mutant plants that defect the common symbiosis signaling pathway exhibited no arbuscule formation. Concerning the reason for this characteristic phenotype of oscerk1, we speculated that OsRLK10, which is a putative paralog of OsCERK1, may have a redundant function in AM symbiosis. The protein sequences of these two genes are highly conserved and it is estimated that the gene duplication occurred 150 million years ago. Here we demonstrated that OsCERK2/OsRLK10 induced AM colonization and chitin-triggered reactive oxygen species production in oscerk1 knockout mutant as similar to OsCERK1. The oscerk2 mutant showed a slight but significant reduction of AM colonization at 5 WAI, indicating the contribution of OsCERK2 for AM symbiosis. However, the oscerk2;oscerk1 double-knockout mutant produced arbuscules at 5 WAI as similar to the oscerk1 mutant, indicating that the redundancy of OsCERK1 and OsCERK2 did not explain the mycorrhizal colonization in oscerk1 at 5 WAI. These results indicated that OsCERK2 has a potential to regulate both chitin-triggered immunity and AM symbiosis and at least partially contributes to AM symbiosis in rice though the contribution of OsCERK2 appears to be weaker than that of OsCERK1.