Printing amphotericin B on microneedles using matrix-assisted pulsed laser evaporation.
Printing amphotericin B on microneedles using matrix-assisted pulsed laser evaporation.
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DOI：
10.18063/ijb.2017.02.004
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发表时间：
2017
影响因子：
8.4
通讯作者：
Narayan RJ
中科院分区：
文献类型：
--
作者：
Sachan R;Jaipan P;Zhang JY;Degan S;Erdmann D;Tedesco J;Vanderwal L;Stafslien SJ;Negut I;Visan A;Dorcioman G;Socol G;Cristescu R;Chrisey DB;Narayan RJ
两性霉素B是一种对真菌和寄生原虫有活性的药物，由于其在生理pH值的水溶液中溶解性差，其经皮给药是一项挑战。在这项研究中，我们使用了一种基于激光的打印方法，即基质辅助脉冲激光蒸发法，将两性霉素B打印在聚乙醇酸微针的表面，这些微针是通过注塑和拉伸光刻相结合的方法制备的。在一种改良的琼脂圆盘扩散试验中，载有两性霉素B的微针对酵母白色念珠菌表现出浓度依赖性的活性。这项研究的结果表明，基质辅助脉冲激光蒸发法可用于在微针表面打印两性霉素B和其他存在复杂溶解性问题的药物。
Transdermal delivery of amphotericin B, a pharmacological agent with activity against fungi and parasitic protozoa, is a challenge since amphotericin B exhibits poor solubility in aqueous solutions at physiologic pH values. In this study, we have used a laser-based printing approach known as matrix-assisted pulsed laser evaporation to print amphotericin B on the surfaces of polyglycolic acid microneedles that were prepared using a combination of injection molding and drawing lithography. In a modified agar disk diffusion assay, the amphotericin B-loaded microneedles showed concentration-dependent activity against the yeast Candida albicans. The results of this study suggest that matrix-assisted pulsed laser evaporation may be used to print amphotericin B and other drugs that have complex solubility issues on the surfaces of microneedles.