Time-dependent enhancement of lymphocyte activation by mitogens after exposure to isolation or water scheduling.
Time-dependent enhancement of lymphocyte activation by mitogens after exposure to isolation or water scheduling.
复制标题
暴露于隔离或水调度后，有丝分裂原对淋巴细胞活化的时间依赖性增强。
DOI：
10.1016/0024-3205(88)90472-9
复制
发表时间：
1988
期刊：
影响因子：
6.1
通讯作者：
Bayer,BM
中科院分区：
文献类型：
--
作者：
Jessop,JJ;Gale,K;Bayer,BM
研究了分离和水分调度对有丝分裂原诱导淋巴细胞增殖的影响。隔离大鼠是指在群体饲养条件下饲养的动物，然后转移到单独的笼子里，在1或2周的时间里自由饮水。饮水计划的大鼠饲养在群舍中（每笼5只大鼠），随机获得食物，但每天有30分钟的饮水时间。将这些组的反应与那些连续圈养的动物进行比较，这些动物可以随意获得食物和水。暴露于分离后1周淋巴细胞对植物血凝素（PHA）的反应无差异。然而，2周后，分离动物的脾脏和血液T淋巴细胞对次优浓度和最大浓度的PHA表现出增加的增殖反应。脾B淋巴细胞对分离动物脂多糖（LPS）的反应也比群养对照组增加了2- 3倍。每天饮水两周后，脾脏淋巴细胞的增殖也同样增加。这种对PHA的反应性增加并没有伴随着淋巴细胞对PHA的敏感性、白细胞总数或脾T淋巴细胞或T辅助淋巴细胞比例的显著变化。我们的研究结果表明，淋巴细胞增殖的增加是时间依赖性的，需要超过1周的隔离暴露，并且是由于对丝裂原或T淋巴细胞数量敏感性变化以外的因素。
The effects of isolation and water scheduling on mitogen induced lymphocyte proliferation were investigated. Isolated rats were animals which had been raised in group-housed conditions and then transferrd to individual cages with ad lib access to water for a 1 or 2 week period. Water scheduled rats were maintained in group housing (5 rats per cage) with ad lib access to food but with access to water for a single 30 minute session each day. Responses of these groups were compared to those of animals which had been continuously group-housed with ad lib access to food and water. No differences in lymphocyte responses to phytohemagglutinin (PHA) were found 1 week after exposure to isolation. However, after 2 weeks, splenic and blood T lymphocytes from isolated animals demonstrated an increased proliferative response to sub-optimum and maximum concentrations of PHA. Splenic B lymphocyte responses to lipopolysaccharide (LPS) from isolated animals were also increased by 2-to 3-fold compared to group-housed controls. Two weeks of exposure of animals to daily water scheduling similarly increased the splenic lymphocyte proliferation. This increased responsiveness to PHA was not accompanied by a significant change in the sensitivity of the lymphocytes to PHA, in the total number of white blood cells, or the proportion of splenic T or T helper lymphocytes. Our results show that the increase in lymphocyte proliferation is time-dependent, requires greater than 1 week of exposure to isolation and is due to factors other than changes in sensitivity to mitogen or T lymphocyte number.