Simultaneous optical and electrical in vivo analysis of the enteric nervous system.
Simultaneous optical and electrical in vivo analysis of the enteric nervous system.
复制标题
肠神经系统的同时光学和电学活体分析。
DOI：
10.1038/ncomms11800
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发表时间：
2016-06-07
影响因子：
16.6
通讯作者：
Shen X
中科院分区：
文献类型：
--
作者：
Rakhilin N;Barth B;Choi J;Muñoz NL;Kulkarni S;Jones JS;Small DM;Cheng YT;Cao Y;LaVinka C;Kan E;Dong X;Spencer M;Pasricha P;Nishimura N;Shen X
肠神经系统（ENS）是神经系统的主要分支，对胃肠道（GI）及其与身体其他部位的沟通至关重要。与大脑和脊髓不同，对ENS的了解相对较少，部分原因是无法直接监测其在活体动物中的活动。在这里，我们集成了一个透明的石墨烯传感器与定制的腹部窗口，同时光学和电气记录的ENS在体内。植入的设备真实的实时捕获ENS对神经递质、药物和光遗传学操作的反应。 肠神经系统（ENS）在调节肠道运动和稳态中起着关键作用，但它仍然是一个具有挑战性的系统。在这里，作者开发了一种新的腹部窗口，允许同时光学和电气记录小鼠ENS系统的活动在较长的时间段。
The enteric nervous system (ENS) is a major division of the nervous system and vital to the gastrointestinal (GI) tract and its communication with the rest of the body. Unlike the brain and spinal cord, relatively little is known about the ENS in part because of the inability to directly monitor its activity in live animals. Here, we integrate a transparent graphene sensor with a customized abdominal window for simultaneous optical and electrical recording of the ENS in vivo. The implanted device captures ENS responses to neurotransmitters, drugs and optogenetic manipulation in real time. The enteric nervous system (ENS) plays a key role in regulating gut motility and homeostasis yet it remains a challenging system to record from. Here, the authors develop a novel abdominal window permitting simultaneous optical and electrical recording of mouse ENS system activity over prolonged time periods.