Real-time imaging of histone H4 hyperacetylation in living cells
Real-time imaging of histone H4 hyperacetylation in living cells
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DOI：
10.1073/pnas.0902150106
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发表时间：
2009-09-22
影响因子：
11.1
通讯作者：
Yoshida, Minoru
中科院分区：
文献类型：
--
作者：
Sasaki, Kazuki;Ito, Tamaki;Yoshida, Minoru
为了可视化活细胞中的组蛋白乙酰化，我们开发了一种基于基因编码的荧光共振能量转移（FRET）的指示剂。指示剂的响应反映了组蛋白 H4 中 K5 和 K8 乙酰化状态的变化。使用这种乙酰化指示剂，我们能够监测有丝分裂期间组蛋白 H4 乙酰化水平的动态波动，以及响应结构不同的组蛋白脱乙酰酶抑制剂而发生的乙酰化变化。
To visualize histone acetylation in living cells, we developed a genetically encoded fluorescent resonance energy transfer ( FRET)based indicator. Response of the indicator reflects changes in the acetylation state of both K5 and K8 in histone H4. Using this acetylation indicator, we were able to monitor the dynamic fluctuation of histone H4 acetylation levels during mitosis, as well as acetylation changes in response to structurally distinct histone deacetylase inhibitors.