GENOTYPIC VARIATION AMONG 6 ISOLATES OF LACTATE DEHYDROGENASE-ELEVATING VIRUS
GENOTYPIC VARIATION AMONG 6 ISOLATES OF LACTATE DEHYDROGENASE-ELEVATING VIRUS
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DOI：
10.1099/0022-1317-67-12-2673
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发表时间：
1986-12-01
影响因子：
3.8
通讯作者：
FERNANDEZ, AV
中科院分区：
文献类型：
--
作者：
BRINTON, MA;GAVIN, EI;FERNANDEZ, AV
本研究采用RNA寡核苷酸指纹图谱分析方法，对6株从近交系小鼠分离的乳酸脱氢酶升高病毒（LDV）进行了比较。四个分离株的基因组RNA给出了独特的指纹图谱。其他两个分离株获得的模式是相似的，但不相同的四个独特的模式之一。这些结果表明，LDV存在一个以上的基因型，病毒分离株可以按基因型分组。我们还通过对3 ''-32 P标记的LDV基因组RNA的直接测序证实了3 ''-末端poly（A）区的存在。3“-末端poly（A）道的存在将LDV与缺乏3”-poly（A）的黄病毒科的成员区分开，并且证明了LDV在披膜病毒科内的当前分类。
In the present study, six lactate dehydrogenase-elevating virus (LDV) isolates obtained independently from inbred mice were compared by RNA oligonucleotide fingerprint analysis. The genome RNAs of four of the isolates gave unique fingerprint patterns. The patterns obtained for the other two isolates were similar, but not identical to one of the four unique patterns. These results indicates that more than one genotype of LDV exists and that virus isolates can be grouped by genotype. We have also demonstrated the presence of a 3''-terminal poly(A) tract by direct sequencing of 3''-32P-labelled LDV genome RNA. The presence of a 3''-terminal poly(A) tract distinguishes LDV from the members of the family Flaviviridae, which lack a 3''-poly(A), and justifies the current classification of LDV within the family Togaviridae.