Anticholinesterase Drugs Stimulate Phosphatidylinositol Response in Rat Tracheal Slices
Anticholinesterase Drugs Stimulate Phosphatidylinositol Response in Rat Tracheal Slices
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抗胆碱酯酶药物刺激大鼠气管切片中的磷脂酰肌醇反应
作者：
O. Shibata;M. Kanairo;Shiping Zhang;H. Hasuo;H. Morooka;T. Fujie;K. Sumikawa
一些抗胆碱酯酶（anti-ChE）药物诱导气道平滑肌收缩。抗胆碱酯酶药物是否刺激气道平滑肌中的毒蕈碱受体以及神经肌肉接头中的烟碱受体尚不清楚。由于磷脂酰肌醇（PI）反应和毒蕈碱激动剂诱导的气道平滑肌收缩之间存在直接关系，我们研究了新斯的明、毒扁豆碱、吡啶斯的明和依地芬铵对气道平滑肌PI反应的影响。将大鼠气管切片置于含有LiCl和[（3）H]肌醇的Krebs-Henseleit溶液中孵育，并加入卡巴胆碱、抗胆碱酯酶或不加。[(3)用液体闪烁计数器计数PI响应的降解产物[3 H]肌醇单磷酸（IP 1）。新斯的明、毒扁豆碱和吡啶斯的明以剂量依赖性方式刺激单磷酸肌醇蓄积，但依地芬铵不影响单磷酸肌醇蓄积。阿托品可完全抑制这些增加。结果表明，新斯的明、毒扁豆碱和吡啶斯的明可刺激气道平滑肌的PI反应，引起支气管收缩，而依洛酚铵不影响PI反应。（Anesth Analg 1996;82：1211-4）
Some anticholinesterase (anti-ChE) drugs induce airway smooth muscle contraction. Whether anti-ChE drugs stimulate muscarinic receptors in airway smooth muscle as well as nicotinic receptors in neuromuscular junction is unknown. Since there is a direct relationship between phosphatidylinositol (PI) response and airway smooth muscle contraction induced by muscarinic agonists, we examined the effects of neostigmine, physostigmine, pyridostigmine, and edrophonium on PI response in the airway smooth muscle. The rat tracheal slices were incubated in Krebs-Henseleit solution containing LiCl and [(3) H]myo-inositol in the presence of carbachol, anti-ChE, or none of them. [(3) H]inositol monophosphate (IP1), which is a degradation product of PI response, was counted with a liquid scintillation counter. Inositol monophosphate accumulation was stimulated by neostigmine, physostigmine, and pyridostigmine in a dose-dependent manner, but was not affected by edrophonium. These increases were completely inhibited by atropine. The results suggest that neostigmine, physostigmine, and pyridostigmine stimulate PI response in the airway smooth muscle, which would cause bronchoconstriction, while edrophonium does not affect PI response. (Anesth Analg 1996;82:1211-4)
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DOI：
10.1164/ajrccm/136.1.43
发表时间：
1987
期刊：
The American review of respiratory disease
影响因子：
--
作者：
Thompson,JE;Scypinski,LA;Gordon,T;Sheppard,D
通讯作者：
Sheppard,D
DOI：
10.1073/pnas.81.21.6899
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BARON, CB;CUNNINGHAM, M;COBURN, RF
通讯作者：
COBURN, RF
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DOI：
--
发表时间：
1985
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Altiere,RJ;Szarek,JL;Diamond,L
通讯作者：
Diamond,L
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DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Park,S;Rasmussen,H
通讯作者：
Rasmussen,H