Spread of drug-resistant pulmonary tuberculosis in Estonia
Spread of drug-resistant pulmonary tuberculosis in Estonia
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DOI：
10.1128/jcm.39.9.3339-3345.2001
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发表时间：
2001-09-01
影响因子：
9.4
通讯作者：
Källenius, G
中科院分区：
文献类型：
--
作者：
Krüüner, A;Hoffner, SE;Källenius, G
对 1994 年从爱沙尼亚新发现的肺结核患者（151 名男性和 58 名女性；平均年龄 41 岁）获得的 209 株结核分枝杆菌临床分离株进行限制性片段长度多态性 (RFLP) 分析表明，61 株分离株 (29%) 属于遗传上密切相关的分离株 A 家族，具有主要的 IS6110 条带模式。这些菌株共享大部分包含 IS6110 DNA 的限制性片段，代表了主要的条带模式（相似性 >65%）。该 A 家族包含 12 个相同分离株的簇，最大的簇包含 10 个菌株。大多数（87.5%）的多重耐药（MDR）分离株，67.2%的所有耐药分离株，但只有12%的完全易感结核分枝杆菌分离株属于A家族。通过spoligotyping证实这些菌株属于北京基因型家族的成员。北京基因型MDR结核分枝杆菌菌株的传播在1997年至1999年也经常出现。结核菌株在很大程度上导致了爱沙尼亚各地耐药结核病的不断出现。
Restriction fragment length polymorphism (RFLP) analysis of 209 Mycobacterium tuberculosis clinical isolates obtained from newly detected pulmonary tuberculosis patients (151 male and 58 female; mean age, 41 years) in Estonia during 1994 showed that 61 isolates (29%) belonged to a genetically closely related group of isolates, family A, with a predominant IS6110 banding pattern. These strains shared the majority of their IS6110 DNA-containing restriction fragments, representing a predominant banding pattern (similarity, >65%). This family A comprised 12 clusters of identical isolates, and the largest cluster comprised 10 strains. The majority (87.5%) of all multidrug-resistant (MDR) isolates, 67.2% of all isolates with any drug resistance, but only 12% of the fully susceptible isolates of M. tuberculosis belonged to family A. These strains were confirmed by spoligotyping as members of the Beijing genotype family. The spread of Beijing genotype MDR M. tuberculosis strains was also frequently seen in 1997 to 1999. The members of this homogenous group of drug-resistant Al. tuberculosis strains have contributed substantially to the continual emergence of drug-resistant tuberculosis all over Estonia.