Evaluation of probiotic characteristics of newly isolated Lactobacillus spp.: Immune modulation and longevity
Evaluation of probiotic characteristics of newly isolated Lactobacillus spp.: Immune modulation and longevity
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DOI：
10.1016/j.ijfoodmicro.2011.05.003
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发表时间：
2011-08-02
影响因子：
5.4
通讯作者：
Whang, Kwang Youn
中科院分区：
文献类型：
--
作者：
Lee, Jin;Yun, Hyun Sun;Whang, Kwang Youn
在本研究中，研究了从韩国婴儿粪便和泡菜中分离的约350株乳酸菌的益生菌潜力。研究了细菌菌株的常见益生菌特性，如耐酸性、耐胆汁性和对人肠上皮细胞（HT-29细胞）的粘附性。一些菌株被发现具有免疫调节和抗菌特性。发现对一组病原菌的拮抗活性是菌株依赖性的。为了评价菌株的免疫调节活性，结合细胞增殖测定淋巴细胞干扰素（IFN）-γ分泌。一些乳酸杆菌菌株产气。发酵乳杆菌和植物乳杆菌表现出IFN-γ水平和淋巴细胞增殖增加。为了评估这些免疫调节乳酸杆菌对宿主寿命的影响，将秀丽隐杆线虫用作体内模型。以热灭活乳酸杆菌为食物源的线虫与以大肠杆菌OP 50为食物源的线虫的寿命有明显差异。用植物乳杆菌CJLP 133和L. 21天后，发酵菌LA 12的抗线虫能力分别比对照线虫提高了13.89%和13.69%（P=0.036和0.043）。此外，还发现了一些安全性特征，包括溶血类型、明胶水合和尿素降解。这些新鉴定的乳酸杆菌有望用作益生菌剂、饲料添加剂和/或食品应用。(C)2011 Elsevier B. V.保留所有权利。
In the current study, the probiotic potential of approximately 350 strains of lactic acid bacteria isolated from Korean infant feces and Kimchi was investigated. Common probiotic properties of the bacterial strains, such as acid tolerance, bile tolerance and adhesion to human intestinal epithelial cells (HT-29 cells), were examined. Some strains were found to have immune modulatory and antimicrobial properties. Antagonistic activity against a panel of pathogenic bacteria was found to be strain dependent. To evaluate the immune modulatory activity of the strains, lymphocyte interferon (IFN)-gamma secretion was determined in conjunction with cell proliferation. Some strains of Lactobacillus gassed. L fermentum and L plantarum exhibited increased IFN-gamma levels and lymphocyte proliferation. To evaluate the effects of these immune modulating lactobacilli on host life span, Caenorhabditis elegans was used as an in vivo model. Nematodes that were supplied heat-killed lactobacilli as a food source exhibited obvious differences in life span compared with those fed Escherichia coli OP50. The mean life span (determined as mean percent survival) of worms fed L plantarum CJLP133 and L. fermentum LA12 was 13.89% and 13.69% greater, respectively, than that of control nematodes after 21 days (P=0.036 and 0.043, respectively). In addition, some of safety profiles, including hemolytic type, gelatin hydration and degradation of urea, were found to be positive. These newly identified lactobacilli hold promise for use as probiotic agents, feed additives and/or in food applications. (C) 2011 Elsevier B.V. All rights reserved.