ONTOGENY OF SPERM SPECIFIC LACTATE DEHYDROGENASE IN MICE
ONTOGENY OF SPERM SPECIFIC LACTATE DEHYDROGENASE IN MICE
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DOI：
10.1002/jez.1401640302
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发表时间：
1967-01-01
影响因子：
--
通讯作者：
HAWTREY, C
中科院分区：
文献类型：
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作者：
GOLDBERG, E;HAWTREY, C
性成熟小鼠睾丸含有一种与精子细胞相关的乳酸脱氢酶同工酶。这种同工酶与其他组织中常见的五种同工酶相比，在亚基组成和对底物DL- -羟基戊酸的反应性方面有所不同。它被称为LDH-X，在标准聚丙烯酰胺凝胶上进行电泳时具有与LDH-5相同的迁移率。这种酶首先出现在睾丸中精母细胞首次成熟的发育过程中。从出生到性成熟，睾丸提取物中LDH的比活性没有明显变化。当老鼠的体重达到6.5至7. 5g睾丸重20 mg，相当于产后15 ~ 17天，LDH-X出现。大约在这个时候，原始生殖细胞（精原细胞）发生第一次分化分裂。在接下来的15-20天内，睾丸以快速的速度生长和分化，在同一时期，精子LDH的比活性也增加。组织化学证据表明，LDH-1，2和3的活性在间质细胞中最高，而LDH-X活性仅限于靠近睾丸小管腔的细胞。
Testes of sexually mature mice (Mus musculus) contain a unique lactate dehydrogenase isozyme associated with the spermatozoan cell. This isozyme differs in subunit composition and reactivity to the substrate DL- -hydroxyvalerate when compared to the usual five isozymes found in o[alpha]ther tissues. It has been termed LDH-X and has the same mobility as LDH-5 when subjected to electro-phoresis on standard polyacrylamide gels. This enzyme first appears during development at the time spermatocytes first mature in the testes. From the time of birth until sexual maturity is reached the specific activity of LDH in testes extracts does not change appreciably. When the mice have reached the weight of 6.5 to 7. 5 g with a testes weight of 20 mg, which is equivalent to an age of 15 to 17 day post-partum, LDH-X appears. It is at approximately this time that the first differentiative division of the primitive germ cell, the sperma-togonium, occurs. For the next period of time, 15-20 days, the testes grow and differentiate at a rapid rate and during this same period the specific activity of the sperm LDH also increases. Histo-chemical evidence indicated that activity of LDH-1, 2, and 3 is highest in the interstitial cells while LDH-X activity is restricted to the cells that are close to the lumen of the testicular tubule.