Electron diffraction provides new information on human stratum corneum lipid organization studied in relation to depth and temperature
Electron diffraction provides new information on human stratum corneum lipid organization studied in relation to depth and temperature
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DOI：
10.1046/j.1523-1747.1999.00706.x
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发表时间：
1999-09-01
影响因子：
6.5
通讯作者：
Koerten, HK
中科院分区：
文献类型：
--
作者：
Pilgram, GSK;Engelsma-van Pelt, AM;Koerten, HK
哺乳动物皮肤的最外层，角质层，为身体提供了防止经表皮水分流失和外界物质渗透的屏障。角质层中角质细胞周围富含脂质的细胞外基质主要负责这种屏障功能。在这项研究中，（冷冻）电子衍射，以获得有关的信息，在细胞外基质中的局部侧向脂质组织在人类角质层的深度。为此目的，从体内和离体天然皮肤制备角质层网格条。结果发现，在室温下制备的样品中的脂质填充主要是正交的。在32 ° C下制备的样品中，在角质层的外层中六边形堆积的存在更明显。逐渐增加的样品温度从30到40摄氏度诱导从斜方晶系到六方亚晶格的进一步过渡，在90摄氏度所有脂质都存在于流体相。这些结果与以前报道的广角X射线衍射和傅里叶变换红外光谱研究结果吻合得很好。我们的结论是，在人类角质层中的脂质是高度有序的整个角质层和电子衍射允许监测局部脂质组织，这有助于了解角质层屏障功能。
The outermost layer of mammalian skin, the stratum corneum, provides the body with a barrier against transepidermal water loss and penetration of agents from outside. The lipid-rich extracellular matrix surrounding the corneocytes in the stratum corneum is mainly responsible for this barrier function. In this study (cryo-) electron diffraction was applied to obtain information about the local lateral lipid organization in the extracellular matrix in relation to depth in human stratum corneum. For this purpose, stratum corneum grid-strips were prepared from native skin in vivo and ex vivo. It was found that the lipid packing in samples prepared at room temperature is predominantly orthorhombic. In samples prepared at 32 degrees C the presence of a hexagonal packing is more pronounced in the outer layers of the stratum corneum. Gradually increasing the specimen temperature from 30 to 40 degrees C induced a further transition from an orthorhombic to a hexagonal sublattice, At 90 degrees C all lipids were present in a fluid phase. These results are in good agreement with previously reported wide angle X-ray diffraction and Fourier transformed infrared spectroscopy studies. We conclude that the lipids in human stratum corneum are highly ordered throughout the stratum corneum and that electron diffraction allows monitoring of the local lipid organization, which contributes to the understanding of stratum corneum barrier function.