Intrinsic mitotic activity supports the human salivary gland acinar cell population
Intrinsic mitotic activity supports the human salivary gland acinar cell population
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DOI：
10.1002/1873-3468.13611
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发表时间：
2019-10-02
期刊：
影响因子：
3.5
通讯作者：
Ovitt, Catherine E.
中科院分区：
文献类型：
--
作者：
Ingalls, Matthew H.;Hollomon, Andrew J.;Ovitt, Catherine E.
为了开发唾液腺功能障碍的治疗方法，重要的是要了解人类唾液腺如何维持正常的稳态。我们实验室先前的数据表明，小鼠唾液腺泡细胞在稳态条件下以及损伤后通过自我复制维持腺泡细胞群。早期研究表明人类腺泡细胞有丝分裂活跃，但所产生的子细胞的身份尚不清楚。使用细胞周期活性和有丝分裂的标记物，以及离体5-乙炔基-2 '-脱氧尿苷测定，我们显示人唾液腺腺泡细胞分裂产生子腺泡细胞。在小鼠中，我们的数据表明，人类唾液腺的稳态是由固有的有丝分裂能力的腺泡细胞。
To develop treatments for salivary gland dysfunction, it is important to understand how human salivary glands are maintained under normal homeostasis. Previous data from our lab demonstrated that murine salivary acinar cells maintain the acinar cell population through self-duplication under conditions of homeostasis, as well as after injury. Early studies suggested that human acinar cells are mitotically active, but the identity of the resultant daughter cells was not clear. Using markers of cell cycle activity and mitosis, as well as an ex vivo 5-Ethynyl-2 '-deoxyuridine assay, we show that human salivary gland acinar cells divide to generate daughter acinar cells. As in mouse, our data indicate that human salivary gland homeostasis is supported by the intrinsic mitotic capacity of acinar cells.