Development and linkage mapping of unigene-derived microsatellite markers in Brassica rapa L.
Development and linkage mapping of unigene-derived microsatellite markers in Brassica rapa L.
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DOI：
10.1270/jsbbs.61.160
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发表时间：
2011-06-01
期刊：
影响因子：
2.4
通讯作者：
Piao, Zhong Yun
中科院分区：
文献类型：
--
作者：
Ge, Yu;Ramchiary, Nirala;Piao, Zhong Yun
油菜是重要的蔬菜、油料和饲料作物。在这里，我们利用从国家生物技术信息中心数据库下载的UNGENES，在白菜中开发了450个Unigene衍生的微卫星(UGMS)标记。在450对UGMS标记中，有428对(95.1%)在至少一个甘蓝型油菜亲本中产生了预期大小的重复性和可靠性的扩增，70个UGMS标记在两个对照亲本之间产生了72个多态位点。对这70个多态UGMS标记在其他5种栽培油菜中的可转移性分析表明，转移率在黑菜型油菜中为82.9%，在芥菜型和甘蓝型油菜中为97.1%，总共有53个UGMS标记在所有5种甘蓝型油菜中扩增了目标。利用72个UGMS多态座位和154个已开发的SSR构建了油菜连锁图谱。本研究新开发的甘蓝型油菜超核不育标记和连锁图谱，将有助于更好地了解甘蓝型油菜基因组与单基因的组织和进化关系，以及重要经济性状的定位、标记和克隆(S)和分子标记辅助育种。
Brassica rapa plants are highly important as vegetables, sources of oilseeds and fodder crop. Here, we developed 450 unigene derived microsatellite (UGMS) markers in B. rapa using unigenes downloaded from the National Center for Biotechnology Information database. Of the 450 UGMS primer pairs, 428 (95.1%) produced repeatable and reliable amplifications of expected size in at least one parental line of B. rapa, and 70 UGMS markers gave 72 polymorphic loci between the two contrasting parental lines. Cross-species transferability analysis of these 70 polymorphic UGMS markers in five other cultivated Brassica species showed varying transferability rates ranging from 82.9% in B. nigra to 97.1% in B. juncea and B. napus, and overall 53 UGMS markers amplified targets in all five species. The B. rapa linkage map was constructed using the 72 UGMS polymorphic loci and 154 previously developed SSRs. The newly developed UGMS markers and linkage map in this study would help in future studies to better understand the organization and evolution of Brassica genomes with respect to unigenes, in addition to mapping, tagging and cloning of economically important trait QTL/gene(s) and marker-assisted breeding in Brassica crops.