Arsenite treatment induces oxidative stress, upregulates antioxidant system, and causes phytochelatin synthesis in rice seedlings
Arsenite treatment induces oxidative stress, upregulates antioxidant system, and causes phytochelatin synthesis in rice seedlings
复制标题
DOI：
10.1007/s00709-010-0210-0
复制
发表时间：
2011-07-01
期刊：
影响因子：
2.9
通讯作者：
Dubey, R. S.
中科院分区：
文献类型：
--
作者：
Mishra, Shruti;Jha, A. B.;Dubey, R. S.
研究了亚砷酸盐处理对两种籼稻活性氧生成、氧化应激诱导、抗氧化系统反应和植物螯合素合成的影响。Malviya-36和Pant-12在沙培养中生长5-20天。亚砷酸盐（As2O3； 25和50 μ M）处理导致超氧阴离子（O(2)）的形成增加。-))， H2O2和硫代巴比妥酸活性物质水平升高，显示脂质过氧化增强。无论脱氢抗坏血酸（DHA）和谷胱甘肽（GSSG）水平的变化如何，都观察到抗坏血酸（AA）和谷胱甘肽（GSH）水平的提高，这反过来影响了AA/DHA和GSH/GSSG的氧化还原比率。随着亚砷酸盐处理的增加，幼苗中酸溶性硫醇和植物螯合素（PC）的合成增加。在抗氧化酶中，亚砷酸盐处理幼苗的超氧化物歧化酶（EC 1.15.1.1）、过氧化氢酶（EC 1.11.1.6）、总抗坏血酸过氧化物酶（APX, EC 1.11.1.11）、叶绿体抗坏血酸过氧化物酶、愈伤木酚过氧化物酶（EC 1.11.1.7）、单脱氢抗坏血酸还原酶（EC 1.6.5.4）、谷胱甘肽还原酶（EC 1.6.4.2）活性升高，脱氢抗坏血酸还原酶（EC 1.8.5.1）活性在5 ~ 10 d内呈下降趋势，之后呈上升趋势。结果表明，亚砷酸盐处理引起水稻幼苗氧化应激，增加了许多酶促和非酶促抗氧化剂的水平，并诱导硫醇和pc的合成，这些物质可能是减轻亚砷酸盐诱导的氧化损伤的重要成分。
The effects of arsenite treatment on generation of reactive oxygen species, induction of oxidative stress, response of antioxidative system, and synthesis of phytochelatins were investigated in two indica rice (Oryza sativa L.) cvs. Malviya-36 and Pant-12 grown in sand cultures for a period of 5-20 days. Arsenite (As2O3; 25 and 50 mu M) treatment resulted in increased formation of superoxide anion (O (2) (.-) ), elevated levels of H2O2 and thiobarbituric acid reactive substances, showing enhanced lipid peroxidation. An enhanced level of ascorbate (AA) and glutathione (GSH) was observed irrespective of the variation in the level of dehydroascorbate (DHA) and oxidized glutathione (GSSG) which in turn influenced redox ratios AA/DHA and GSH/GSSG. With progressive arsenite treatment, synthesis of total acid soluble thiols and phytochelatins (PC) increased in the seedlings. Among antioxidative enzymes, the activities of superoxide dismutase (EC 1.15.1.1), catalase (EC 1.11.1.6), total ascorbate peroxidase (APX, EC 1.11.1.11), chloroplastic ascorbate peroxidase, guaiacol peroxidase (EC 1.11.1.7), monodehydroascorbate reductase (EC 1.6.5.4), and glutathione reductase (EC 1.6.4.2) increased in arsenite treated seedlings, while dehyroascorbate reductase (EC 1.8.5.1) activity declined initially during 5-10 days and increased thereafter. Results suggest that arsenite treatment causes oxidative stress in rice seedlings, increases the levels of many enzymatic and non-enzymatic antioxidants, and induces synthesis of thiols and PCs, which may serve as important components in mitigating arsenite-induced oxidative damage.