A structural model for microtubule minus-end recognition and protection by CAMSAP proteins.
A structural model for microtubule minus-end recognition and protection by CAMSAP proteins.
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DOI：
10.1038/nsmb.3483
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发表时间：
2017-11
影响因子：
16.8
通讯作者：
Akhmanova A
中科院分区：
文献类型：
--
作者：
Atherton J;Jiang K;Stangier MM;Luo Y;Hua S;Houben K;van Hooff JJE;Joseph AP;Scarabelli G;Grant BJ;Roberts AJ;Topf M;Steinmetz MO;Baldus M;Moores CA;Akhmanova A
CAMSAP和Patronin家族成员调节微管负端的稳定性和定位，从而形成对细胞分裂、极化和分化至关重要的非中心体微管网络。在这里，我们发现CAMSAPs的CKK结构域广泛存在于真核生物中，并自主识别微管负端。通过结构方法的结合，我们揭示了哺乳动物CKK是如何在外微管表面原丝间界面的两个微管蛋白二聚体之间结合的。结合高分辨率荧光显微镜和冷冻电子断层扫描的体外重建分析表明，CKK优先结合在弯曲的原丝和规则的微管晶格之间的过渡区。我们认为，该位点原细丝间界面的负端特异性特征构成了CKK的负端偏好的基础。微管结合的CKK和Kinesin马达之间的空间碰撞解释了CKK如何保护微管负端免受Kinesin-13诱导的解聚，从而控制游离微管负端的稳定性。
CAMSAP and Patronin family members regulate microtubule minus-end stability and localization and thus organize non-centrosomal microtubule networks, which are essential for cell division, polarization and differentiation. Here, we show that the C-terminal CKK domain of CAMSAPs is widely present among eukaryotes and autonomously recognizes microtubule minus ends. Through a combination of structural approaches, we uncover how mammalian CKK binds between two tubulin dimers at the inter-protofilament interface on the outer microtubule surface. In vitro reconstitution assays combined with high resolution fluorescence microscopy and cryo-electron tomography suggest that CKK preferentially associates with the transition zone between curved protofilaments and the regular microtubule lattice. We propose that minus-end-specific features of the inter-protofilament interface at this site form the basis for CKK’s minus-end preference. The steric clash between microtubule-bound CKK and kinesin motors explains how CKK protects microtubule minus ends against kinesin-13-induced depolymerization and thus controls the stability of free microtubule minus ends.
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影响因子：
14.9
作者：
Finn RD;Clements J;Arndt W;Miller BL;Wheeler TJ;Schreiber F;Bateman A;Eddy SR
通讯作者：
Eddy SR
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
10.7
作者：
Baines, Anthony J.;Bignone, Paola A.;Phillips, Gareth W.
通讯作者：
Phillips, Gareth W.
影响因子：
1.7
作者：
Baldus, M;Petkova, AT;Griffin, RG
通讯作者：
Griffin, RG
影响因子：
5.7
作者：
Chretien, D;Kenney, JM;Wade, RH
通讯作者：
Wade, RH