Selected indigestible oligosaccharides affect large bowel mass, cecal and fecal short-chain fatty acids, pH and microflora in rats
Selected indigestible oligosaccharides affect large bowel mass, cecal and fecal short-chain fatty acids, pH and microflora in rats
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DOI：
10.1093/jn/127.1.130
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发表时间：
1997-01-01
影响因子：
4.2
通讯作者：
Wolf, BW
中科院分区：
文献类型：
--
作者：
Campbell, JM;Fahey, GC;Wolf, BW
某些难消化的低聚糖可通过理想细菌种类的发酵和增殖而有益于胃肠道健康。本研究的目的是阐明所选寡糖对大鼠盲肠和粪便短链脂肪酸（SCFA）浓度、pH值、总大肠湿重和壁重以及肠道微生物群水平的影响。将50只雄性Sprague-Dawley大鼠随机分配至5种处理之一：1）对照饮食; 2）对照饮食+5%微晶纤维素（5%CC）; 3）对照饮食+5%CC +6%低聚果糖; 4）对照饮食+5%CC +6%低聚果糖;或5）对照饮食+5%CC +6%低聚木糖。对照饮食由（基于干物质）20%蛋白质、65%碳水化合物、10.5%脂肪、维生素和矿物质混合物组成。试验时间为14 d。低聚果糖和低聚果糖的饮食导致更高的盲肠丁酸浓度与控制，纤维素和低聚木糖的饮食。一般来说，总盲肠SCFA池较高，而pH值较低，从ingredianooligosaccharides含有饮食相比，控制或纤维素饮食。盲肠总重量和壁重量较高，从低聚糖消费，而结肠总湿重较高的大鼠消耗低聚木糖相比，其他治疗;结肠壁重量不受治疗。与喂食对照饮食的大鼠相比，喂食低聚糖饮食的大鼠盲肠生物多样性细菌和总厌氧菌较高，而总需氧菌较低。盲肠乳酸杆菌水平不受给药影响。通过提供SCFA、降低pH值和增加双歧杆菌，在饮食中掺入可发酵、不可消化的低聚糖可能有益于改善胃肠道健康。
Certain indigestible oligosaccharides may benefit gastrointestinal tract health via fermentation and proliferation of desirable bacterial species. The purpose of this study was to elucidate effects of selected oligosaccharides on cecal and fecal short-chain fatty acid (SCFA) concentration, pH, total large bowel wet weight and wall weight, and gut microbiota levels in rats. Fifty male Sprague-Dawley rats were randomly assigned to one of five treatments: 1) control diet; 2) control diet + 5% microcrystalline cellulose (5% CC); 3) control diet + 5% CC + 6% fructooligosaccharides; 4) control diet + 5% CC + 6% oligofructose; or 5) control diet + 5% CC + 6% xylooligosaccharides. The control diet consisted of (dry matter basis) 20% protein, 65% carbohydrate, 10.5% fat, vitamin and mineral mixes. The duration of the study was 14 d. The oligofructose- and fructooligosaccharide-containing diets resulted in higher cecal butyrate concentrations compared with the control, cellulose and xylooligosaccharide diets. Generally, total cecal SCFA pools were higher while pH was lower from ingesting oligosaccharide-containing diets compared with control or cellulose diets. Cecal total weight and wall weight were higher from oligosaccharide consumption, whereas colonic total wet weight was higher for rats consuming xylooligosaccharides compared with other treatments; colon wall weight was unaffected by treatments. Cecal bifidobacteria and total anaerobes were higher whereas total aerobes were lower in rats fed oligosaccharide diets compared with those fed the control diet. Cecal lactobacilli levels were unaffected by treatment. Dietary incorporation of fermentable, indigestible oligosaccharides, by providing SCFA, lowering pH, and increasing bifidobacteria, may be beneficial in improving gastrointestinal health.