Chemical and cellular antioxidant activity of two novel peptides designed based on glutathione structure
Chemical and cellular antioxidant activity of two novel peptides designed based on glutathione structure
复制标题
基于谷胱甘肽结构设计的两种新型肽的化学和细胞抗氧化活性
DOI：
10.1016/j.fct.2012.08.028
复制
发表时间：
2012-11-01
影响因子：
4.3
通讯作者：
Ren, Jiaoyan
中科院分区：
文献类型：
--
作者：
Gu, Longjian;Zhao, Mouming;Ren, Jiaoyan
以谷胱甘肽（Glu-Cys-Gly，GSH）为基础，设计了两种新型多肽ECH（Glu-Cys-His）和YECG（Tyr-Glu-Cys-Gly），并对其抗氧化活性进行了研究。采用单电子转移（SET）和氢原子转移（HAT）等多种化学方法对多肽的抗氧化活性进行了评价。在SET法中，ECH三肽的DPPH自由基清除率最高（80.16%），还原力最强（A（700）= 0.378）。此外，ECH对亚油酸过氧化反应的抑制活性最强，第7天的抑制率为98.25%。然而，对于另外两个基于HAT的测定，四肽YECG显示出非凡的氧自由基吸收能力（ORAC值= 2.42 μ M Trolox/μ M）和ABTS自由基清除能力（8.88 mM Trolox/mM）。体外培养的PC 12细胞模型也表明，YECG对PC 12细胞抗H2 O2损伤的保护作用最强。结果表明，抗氧化氨基酸（His/Tyr）的存在及其在肽链中的位置是导致ECH和YECG抗氧化能力差异的主要原因。His位于C末端位置。ECH表现出良好的供电子能力，而YECG在N-末端具有Tyr，表现出较强的氧自由基吸收能力。(C)2012爱思唯尔有限公司保留所有权利。
Two novel peptides, ECH (Glu-Cys-His) and YECG (Tyr-Glu-Cys-Gly), were designed based on glutathione (Glu-Cys-Gly, GSH) and their antioxidant activities were studied. Various chemical methods based on single-electron-transfer (SET) and hydrogen-atom-transfer (HAT) were applied to evaluate the antioxidant activities of the peptides. For SET-based assay, tripeptide ECH displayed the highest DPPH radical scavenging activity (80.16%) and the strongest reducing power (A(700) = 0.378). Besides, ECH also exhibited the best inhibition activity toward linoleic acid peroxidation with inhibition rate 98.25% at 7th day, which is a HAT-based assay. However, for another two HAT-based assays, it was tetrapeptide YECG that showed extraordinary oxygen radical absorption capacity (ORAC value = 2.42 mu M Trolox/mu M) and ABTS free radical scavenging ability (8.88 mM Trolox/mM). In vitro cultured PC12 cell model also suggested that YECG gave the best protection for PC12 cells to resist H2O2-treated necrosis. It was found that the discrepancy of antioxidant capacity between ECH and YECG was caused by the presence of antioxidant amino acids (His/Tyr) and their position in peptide chain. With His located at C-terminal position. ECH demonstrated good electrons donating capacity, while with Tyr at N-terminal position, YECG exhibited strong oxygen radical absorbance capacity. (C) 2012 Elsevier Ltd. All rights reserved.