Distinct evolutionary origins of common multi-drug resistance phenotypes in Salmonella typhimurium DT104: a convergent process for adaptation under stress
Distinct evolutionary origins of common multi-drug resistance phenotypes in Salmonella typhimurium DT104: a convergent process for adaptation under stress
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DOI：
10.1007/s00438-019-01531-5
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发表时间：
2019-02
影响因子：
3.1
通讯作者：
Le Tang;Songling Zhu;X. Fang;Yong-Guo Li;C. Poppe;R. Johnston;Guirong Liu;Shu-Lin Liu
中科院分区：
文献类型：
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作者：
Le Tang;Songling Zhu;X. Fang;Yong-Guo Li;C. Poppe;R. Johnston;Guirong Liu;Shu-Lin Liu
抗生素耐药性使病原菌难以控制，但对耐药性获得或丧失的一般过程知之甚少。在这里，我们比较了不同的S。对0种、2种、5种或更多种供试抗菌剂耐药的鼠伤寒DT 104菌株。我们发现，共同的抗性表型可以由不同的基因编码，SGI-1或质粒。我们还证明了所有测试的非耐药和不同耐药的DT 104菌株之间的紧密克隆性，证明了遗传性状的动态获得或丢失（通过多药耐药基因簇的完全缺失或逐渐衰减）。这些发现反映了通过从随机来源获得所需性状来使细菌对多种抗菌剂具有抗性的趋同过程。当抗菌素胁迫不存在时，耐药基因可能会迅速脱落，因此细菌可以节省维持不需要的基因的成本。因此，这项工作重申了严格控制抗菌药物使用的重要性。
Antimicrobial resistance makes pathogenic bacteria hard to control, but little is known about the general processes of resistance gain or loss. Here, we compared distinct S. typhimurium DT104 strains resistant to zero, two, five, or more of the tested antimicrobials. We found that common resistance phenotypes could be encoded by distinct genes, on SGI-1 or plasmid. We also demonstrated close clonality among all the tested non-resistant and differently resistant DT104 strains, demonstrating dynamic acquisition or loss (by total deletion or gradual decaying of multi-drug resistance gene clusters) of the genetic traits. These findings reflect convergent processes to make the bacteria resistant to multiple antimicrobials by acquiring the needed traits from stochastically available origins. When the antimicrobial stress is absent, the resistance genes may be dropped off quickly, so the bacteria can save the cost for maintaining unneeded genes. Therefore, this work reiterates the importance of strictly controlled use of antimicrobials.