MonoRes: Automatic and Accurate Estimation of Local Resolution for Electron Microscopy Maps
MonoRes: Automatic and Accurate Estimation of Local Resolution for Electron Microscopy Maps
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DOI：
10.1016/j.str.2017.12.018
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发表时间：
2018-02-06
期刊：
影响因子：
5.7
通讯作者：
Sorzano, Carlos Oscar S.
中科院分区：
文献类型：
--
作者：
Luis Vilas, Jose;Gomez-Blanco, Josue;Sorzano, Carlos Oscar S.
自电子显微镜出现以来，分辨率一直是一个关键参数。在本文中，我们提出了一种全自动、精确的方法来确定三维地图的局部分辨率（MonoRes）。该算法的基础是分析信号概念的扩展，称为单基因信号。在不同频率下对地图进行滤波，计算单基因信号的幅度，然后应用一个准则来确定每个体素的分辨率。MonoRes是全自动的，无需强制用户参数，在所有测试中都具有很高的准确性，并且在计算上比该领域现有的方法更快。此外，MonoRes还提供了基于计算出的局部分辨率对原始地图进行局部滤波的选项。
Since the beginning of electron microscopy, resolution has been a critical parameter. In this article, we propose a fully automatic, accurate method for determining the local resolution of a 3D map (MonoRes). The foundation of this algorithm is an extension of the concept of analytic signal, termed monogenic signal. The map is filtered at different frequencies and the amplitude of the monogenic signal is calculated, after which a criterion is applied to determine the resolution at each voxel. MonoRes is fully automatic without compulsory user parameters, with great accuracy in all tests, and is computationally more rapid than existing methods in the field. In addition, MonoRes offers the option of local filtering of the original map based on the calculated local resolution.