The Escherichia fergusonii iucABCD iutA genes are located within a larger chromosomal region similar to pathogenicity Islands.
The Escherichia fergusonii iucABCD iutA genes are located within a larger chromosomal region similar to pathogenicity Islands.
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弗格森埃希氏菌 iucABCD iutA 基因位于与致病性岛类似的更大的染色体区域内。
DOI：
10.1007/bf02930946
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发表时间：
2003
影响因子：
2.6
通讯作者：
Weinstock,GM
中科院分区：
文献类型：
--
作者：
Smajs,D;Smarda,J;Weinstock,GM
在50个菌株中，有3个菌株（EF 873，EF 1496，EF 939）产氧肟酸铁载体。筛选菌株EF 873染色体DNA的粘粒文库（在不产生需氧肌动蛋白的大肠杆菌VCS 257中），以鉴定产需氧肌动蛋白的大肠杆菌ABCDandiutA基因直系同源物。预测的IucABCD和IutA蛋白与福氏志贺菌和大肠杆菌的相应蛋白具有59-65%的同源性。杆菌产气杆菌素分子由E. fergusoniandE.大肠杆菌刺激携带E. coliorE. fergusonii iutAgenes.在iucandiut基因上游12 kb和下游17 kb区域，鉴定出20个额外的ORF。它们的基因产物弗罗姆. coli、S.福氏杆菌、产气克雷伯菌、铜绿假单胞菌和霍乱弧菌。识别25 kb以上DNA序列的探针，包括iucABC和diutA基因，与两个产气杆菌素菌株（EF 873和EF 939）的染色体DNA杂交，但不与其他非产气杆菌素菌株杂交。Fergusoniisstrains测试。这些数据，连同该区域的遗传组织，表明E。fergusonii iucABCD iutA基因是类似于其它细菌的致病岛的较大DNA片段的一部分。
Three strains ofEscherichia fergusonii(EF873, EF1496, EF939) of 50 strains tested produced the hydroxamate siderophore aerobactin. Screening of a cosmid library of the strain EF873 chromosomal DNA (in aerobactin nonproducingEscherichia coliVCS257) for aerobactin production identifiediucABCDandiutAgene orthologues. The predicted IucABCD and IutA proteins showed 59–65% identity to the corresponding proteins ofShigella flexneriandE. coli. Aerobactin molecules synthesized byE. fergusoniiandE. colistrains stimulated growth of aerobactin indicator strains harboring eitherE. coliorE. fergusonii iutAgenes. In the 12 kb upstream and 17 kb downstream regions of theiucandiutgenes, 20 additional ORFs were identified. Their gene products showed homology to proteins fromE. coli, S. flexneri, Klebsiella aerogenes, Pseudomonas aeruginosaandVibrio cholerae. Probes recognizing DNA sequences from a region of more than 25 kb, which included theiucABCDandiutAgenes, hybridized with chromosomal DNA of two aerobactin-producing strains (EF873 and EF939), but not with other nonproducingE. fergusoniistrains tested. These data, together with the genetic organization of this region, suggest thatE. fergusonii iucABCD iutAgenes are a portion of a larger segment of DNA similar to pathogenicity islands of other bacteria.