Membrane-dependent uridylylation of the genome-linked protein VPg of poliovirus.
Membrane-dependent uridylylation of the genome-linked protein VPg of poliovirus.
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脊髓灰质炎病毒基因组连锁蛋白 VPg 的膜依赖性尿苷酰化。
DOI：
10.1073/pnas.80.24.7447
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发表时间：
1983
影响因子：
11.1
通讯作者：
Wimmer,E
中科院分区：
文献类型：
--
作者：
Takegami,T;Kuhn,RJ;Anderson,CW;Wimmer,E
在脊髓灰质炎病毒感染的HeLa细胞的膜片段中体外合成了一个小的核苷酸蛋白。对核苷酸和多肽的分析表明，该核苷酸基蛋白为VPg-pUpU：脊髓灰质炎病毒RNA的基因组连接蛋白，共价结合于脊髓灰质炎病毒RNA的前两个5'端核苷酸。体外合成的VPg-pUpU对非离子洗涤剂敏感。我们认为VPg-pUpU是脊髓灰质炎病毒RNA在膜环境中复制起始复合物的一部分。
A small nucleotidyl-protein has been synthesized in vitro in a membrane fraction of poliovirus-infected HeLa cells. Analyses of the nucleotides and polypeptide have shown that the nucleotidyl-protein is VPg-pUpU: the genome-linked protein of poliovirion RNA covalently bound to the first two 5'-terminal nucleotides of poliovirus RNA. Synthesis of VPg-pUpU in vitro was sensitive to nonionic detergent. We suggest that VPg-pUpU is part of the initiation complex in poliovirus RNA replication in a membranous environment.