The nucleocapsid protein of measles virus blocks host interferon response.
The nucleocapsid protein of measles virus blocks host interferon response.
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DOI：
10.1016/j.virol.2011.12.011
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发表时间：
2012-03
期刊：
影响因子：
3.7
通讯作者：
I. Takayama;Hiroki Sato-;A. Watanabe;Mio Omi-Furutani;A. Sugai;K. Kanki;M. Yoneda;C. Kai
中科院分区：
文献类型：
--
作者：
I. Takayama;Hiroki Sato-;A. Watanabe;Mio Omi-Furutani;A. Sugai;K. Kanki;M. Yoneda;C. Kai
麻疹病毒（MV）属于副粘病毒科的麻疹病毒属。许多副粘病毒通过各种机制抑制宿主免疫系统中的宿主干扰素（IFN）信号传导途径。已经描述了许多副粘病毒的抑制机制。虽然有不一致的以前的报告中关于MV，它似乎是P/V/C蛋白干扰的途径。本研究证实了MV野毒株P基因产物对IFN途径的影响，并检测了其他病毒蛋白对IFN途径的影响，有趣的是，我们发现N蛋白作为IFN-α/β和γ-拮抗剂的作用与P基因产物一样强。我们进一步研究了MV-N抑制的机制，发现MV-N阻断活化的STAT的核输入而不阻止STAT和Jak的活化或STAT降解，并且MV-N的核转位对于抑制是重要的。观察到N蛋白的抑制作用是其他麻疹病毒的共同特征。本研究结果表明，MV的N蛋白和P/V/C蛋白参与了对宿主IFN信号通路的抑制。
Measles virus (MV) belongs to the genus Morbillivirus of the family Paramyxoviridae. A number of paramyxoviruses inhibit host interferon (IFN) signaling pathways in host immune systems by various mechanisms. Inhibition mechanisms have been described for many paramyxoviruses. Although there are inconsistencies among previous reports concerning MV, it appears that P/V/C proteins interfere with the pathways. In this study, we confirmed the effects of MV P gene products of a wild MV strain on IFN pathways and examined that of other viral proteins on it. Interestingly, we found that N protein acts as an IFN-α/β and γ-antagonist as strong as P gene products. We further investigated the mechanisms of MV-N inhibition, and revealed that MV-N blocks the nuclear import of activated STAT without preventing STAT and Jak activation or STAT degradation, and that the nuclear translocation of MV-N is important for the inhibition. The inhibitory effect of the N protein was observed as a common feature of other morbilliviruses. The results presented in this report suggest that N protein of MV as well as P/V/C proteins is involved in the inhibition of host IFN signaling pathways.