ULTRASTRUCTURAL ANALYSIS OF INDWELLING VASCULAR CATHETERS - A QUANTITATIVE RELATIONSHIP BETWEEN LUMINAL COLONIZATION AND DURATION OF PLACEMENT
ULTRASTRUCTURAL ANALYSIS OF INDWELLING VASCULAR CATHETERS - A QUANTITATIVE RELATIONSHIP BETWEEN LUMINAL COLONIZATION AND DURATION OF PLACEMENT
复制标题
DOI：
10.1093/infdis/168.2.400
复制
发表时间：
1993-08-01
影响因子：
6.4
通讯作者：
BODEY, GP
中科院分区：
文献类型：
--
作者：
RADD, I;COSTERTON, W;BODEY, GP
为了评估长期中心静脉导管 (CVC) 的腔内和腔外定植程度，对来自 340 名连续癌症患者的 359 根留置硅胶 CVC 进行了检查。 All CVC were cultured by the roll-plate and sonication quantitative culture techniques. Semiquantitative electron microscopy was done on 39 CVC associated with catheter infections and on 26 culture-negative controls. An additional 10 culture-negative CVC obtained after death were also studied by electron microscopy. Ultrastructural colonization and biofilm formation was universal and quantitatively independent of clinical catheter-related infections. Ultrastructural colonization and biofilm formation was predominantly luminal in long-term CVC (>30 days).根据综合定义，滚板导管尖端培养的敏感性为 42%-45%，而尖端超声处理的敏感性为 65%-72%。 Colonization of indwelling catheters is universal regardless of culture results.对于长期 CVC，定植主要发生在管腔内，管腔外定量导管培养的诊断敏感性有限。
To assess the degree of luminal and extraluminal colonization of long-term central venous catheters (CVC), 359 indwelling silicone CVC from 340 consecutive cancer patients were examined. All CVC were cultured by the roll-plate and sonication quantitative culture techniques. Semiquantitative electron microscopy was done on 39 CVC associated with catheter infections and on 26 culture-negative controls. An additional 10 culture-negative CVC obtained after death were also studied by electron microscopy. Ultrastructural colonization and biofilm formation was universal and quantitatively independent of clinical catheter-related infections. Ultrastructural colonization and biofilm formation was predominantly luminal in long-term CVC (>30 days). Based on a composite definition, the sensitivity of the roll-plate catheter tip culture was 42%-45% compared with 65%-72% for the sonication of the tip. Colonization of indwelling catheters is universal regardless of culture results. For long-term CVC, colonization becomes predominantly luminal and extraluminal quantitative catheter cultures are of limited diagnostic sensitivity.