Isolation of H3N2 swine influenza virus in South Korea
Isolation of H3N2 swine influenza virus in South Korea
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DOI：
10.1177/104063870301500107
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发表时间：
2003-01-01
影响因子：
1.5
通讯作者：
Park, BK
中科院分区：
文献类型：
--
作者：
Song, DS;Lee, JY;Park, BK
猪流感是一种重要的呼吸道疾病，偶尔会导致幼稚猪群的繁殖问题。虽然不同亚型的猪流感病毒(SIV)与亚洲国家的猪流感临床暴发有关，但尚未在韩国猪群中分离出H1N1亚型以外的亚型SIV。1998年12月，在韩国庆济省的一个商业养猪场发现了急性呼吸道疾病的暴发。从指标猪群中的3头仔猪的肺中检测到一种凝集公鸡红细胞的病原体，并使用商品化流感病毒分型试剂盒确定为A型流感病毒。猪抗H3N2SIV分离株(A/Sw/IA/41305/1998)的血凝活性可被猪抗血清完全抑制，但不能被H1N1猪抗血清(A/Sw/IA/1979)所抑制。逆转录-聚合酶链式反应(RT-PCR)显示3株均为H3SIV亚型。血凝素基因聚合酶链式反应产物的序列分析支持韩国H3SIV分离株在基因上与已知的哺乳动物H3型流感病毒相似的观点。这是韩国首次报告由H3N2型病毒引起的猪流感临床暴发。
Swine influenza is a significant respiratory disease causing occasional reproductive problems in naive swine herds. Although different subtypes of swine influenza virus (SIV) have been implicated in clinical outbreaks of swine influenza in Asian countries, no virus isolation has been made to identify SIV of subtypes other than the H1N1 subtype in the Korean swine population. In December 1998, an outbreak of acute respiratory disease was identified in a commercial swine farm located in the Kyunggi province of South Korea. A causative agent, which agglutinated rooster red blood cells, was detected from the lungs of 3 piglets from the index herd and was determined to be type A influenza virus using a commercial influenza virus typing kit. Hemagglutination activity (HA) of the isolates was completely inhibited by a swine antiserum against a recent US H3N2 SIV isolate (A/Sw/IA/41305/1998) but not by H1N1 swine antiserum (A/Sw/IA/1979). Reverse transcription-polymerase chain reaction (RT-PCR) revealed all 3 isolates were H3 SIV subtypes. Sequence analysis of hemagglutinin gene PCR products supported the belief that the Korean H3 SIV isolates were genetically similar to the known mammalian H3 influenza viruses. This is the first report on a clinical outbreak of swine influenza caused by the H3N2 virus in Korea.